Hematopoietic toxicity in Wistar rats experimentally exposed to cement dust

This study investigates the pattern of CD hematopoietic effect using a controlled model of exposure. Thirty (30 ) 3-month-old male Wistar rats weighing between 150 and 180g were randomized into three (3) groups (n = 10); control, 14-day and 28-day exposed. We assessed hematological differentials and indices, osmotic fragility test, erythrocyte morphology, and morphometry. Gross osteological variables and indices, osteohistopathology, and bone marrow cytology were also investigated. Results show that CD a lters hematological variables with significant increase in packed cell volume (PCV), erythrocyte count (RBC), hemoglobin (Hb), erythrocyte sedimentation rate (ESR), lymphocyte, and reticulocyte count with a significant reduction in neutrophil count. Abnormal erythromorphology and morphometry observe d significantly differ from the control. We found a significantly higher osmotic concentration-independent fragility. Terminal body weight and various osteological parameters including weight/foraminal index ratio (WFiR), relative tuberosity (ReT), relative epicondyle (ReE), relative femoral width (RFW), relative femoral length (RFL), ponderal, and bone density indices significantly differ from control. Osteohistopathological assessment of the exposed groups shows an array of alterations in the trabecula morphology and marrow cavity. The retinue of data from bone marrow cytology reveals that the bone internal matrix appears necrotic with dispersed cellular debris. The availabl...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research