Dose and Time Response Study to Develop Retinal Degenerative Model of Zebrafish with Lead Acetate

This study was designed to calculate the lowest possible dose of PbAc (lead acetate), a neurotoxicant, that can have a toxicological impact on the zebrafish retina and to minimize animal usage. Dose and time-dependent changes were observed in the zebrafish retina following PbAc exposure with zero mortality. Vision and visual behavior response are the foremost indicators that can be recorded to mark the risk assessment of any chemical. Therefore, the present study aims at dose and time response to find the lowest dose of PbAc affecting the zebrafish retina and its visual behavior. Zebrafish were treated for 3 weeks with four concentrations of 0.04, 0.06, 0.08, and 0.1 mg/L of PbAc for a dose-response study. Then for the time response study, two doses 0.08 and 0.1 mg/L were selected and zebrafish were exposed to those concentrations for 2 and 4 weeks. The results of qualitative and quantitative analyses of retinal histology showed that 15 days of treatment with 0.08mg/L concentration can cause appropriate damage to the photoreceptor layer. At the ultrastructural level, it was further observed that PbAc induces damage to the photoreceptors, especially the rod cells. Escape response behavior showed a significant decrease in visual response to changing contrasts in an increasing dose-dependent manner.PMID:34706603 | DOI:10.1080/15569527.2021.1999254
Source: Cutaneous and Ocular Toxicology - Category: Toxicology Authors: Source Type: research