The Feasibility Study of Plasma-activated Water as Physical Therapy to Induce Apoptosis in ...

In this study, a Helium (He) plasma jet operating in 0.75 W dissipated power and 15 kHz frequency was used as cold atmospheric plasma source. The electrical, thermal, and optical properties of the (He) plasma jet, as well as the pH, conductivity, and temperature of the PAW samples, Low-temperature plasma (LTP) has demonstrated great potential in biomedicine, especially in cancer therapy in-vivo and in-vitro. Plasma activated water (PAW) as an indirect plasma therapy is a significant source of reactive oxygen and nitrogen species (RONS) which play an important role in apoptosis induction in cancer cells. In this study, Helium (He) plasma jet operating in 0.75 W and 20 kHz as dissipated power and frequency, respectively, is used as the cold plasma source. The electrical, thermal, and spectroscopic properties of (He) plasma jet and pH as well as the conductivity and temperature of PAW samples, are investigated. The concentration of hydrogen peroxide (H2O2), nitrite (NO2-) and nitrate (NO-3), which are produced in water as long-lived anticancer RONS, was measured 471.6, 7.9 and 93.5μM, respectively after 6 min of plasma treatment. Moreover, there are descriptions of the mechanism behind the production of the secondary reactive species in air and water. Alamar Blue and flow cytometry assays were employed to investigate the B16F10 cancer metabolic activity and apoptosis. These data support that cold atmospheric plasma (CAP) can produce a certain concentration of anti-cancer agen...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research