Cancers, Vol. 13, Pages 4781: Heterogeneity of Melanoma Cell Responses to Sleep Apnea-Derived Plasma Exosomes and to Intermittent Hypoxia

Cancers, Vol. 13, Pages 4781: Heterogeneity of Melanoma Cell Responses to Sleep Apnea-Derived Plasma Exosomes and to Intermittent Hypoxia Cancers doi: 10.3390/cancers13194781 Authors: Abdelnaby Khalyfa Wojciech Trzepizur Alex Gileles-Hillel Zhuanhong Qiao David Sanz-Rubio José M. Marin Miguel A. Martinez-Garcia Francisco Campos-Rodriguez Isaac Almendros Ramon Farre Manuel Sanchez-de-la-Torre Francisco García-Río David Gozal Obstructive sleep apnea (OSA) is associated with increased cutaneous melanoma incidence and adverse outcomes. Exosomes are secreted by most cells, and play a role in OSA-associated tumor progression and metastasis. We aimed to study the effects of plasma exosomes from OSA patients before and after adherent treatment with continuous positive airway pressure (CPAP) on melanoma cells lines, and also to identify exosomal miRNAs from melanoma cells exposed to intermittent hypoxia (IH) or normoxia. Plasma-derived exosomes were isolated from moderate-to-severe OSA patients before (V1) and after (V2) adherent CPAP treatment for one year. Exosomes were co-incubated with three3 different melanoma cell lines (CRL 1424; CRL 1619; CRL 1675) that are characterized by genotypes involving different mutations in BRAF, STK11, CDKN2A, and PTEN genes to assess the effect of exosomes on cell proliferation and migration, as well as on pAMK activity in the presence or absence of a chemical activator. Subsequently, CRL-1424 and CRL-1675 cells we...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research