miR ‑125a‑5p reverses epithelial‑mesenchymal transition and restores drug sensitivity by negatively regulating TAFAZZIN signaling in breast cancer

Mol Med Rep. 2021 Nov;24(5):812. doi: 10.3892/mmr.2021.12452. Epub 2021 Sep 22.ABSTRACTMicroRNA (miR)‑125a‑5p represses tafazzin phospholipid‑lysophospholipid transacylases (TAFAZZIN) expression and inhibits the epithelial‑mesenchymal transition (EMT) of ovarian cancer cells. EMT was found to have a crucial role in the acquisition of chemoresistance. Thus, the present study aimed to determine whether miR‑125a‑5p reverses EMT and restores drug sensitivity by negatively regulating TAFAZZIN in breast cancer. The expression of miR‑125a‑5p/TAFAZZIN and its association with chemotherapy response were determined in tissue samples from patients with breast cancer. Furthermore, the effects of miR‑125a‑5p on breast cancer cells were elucidated using cell proliferation and cell apoptosis assays. Then, the regulatory mechanism of miR‑125a‑5p in breast cancer was investigated by reverse transcription‑quantitative PCR, western blotting, dual‑luciferase reporter and RNA immunoprecipitation assays. The results demonstrated that miR‑125a‑5p inhibited the EMT of MCF‑7/adriamycin (Adr) breast cancer cells, as well as decreased the proliferation and increased the apoptosis of breast cancer cells treated with Adr/docetaxel. In addition, miR‑125a‑5p downregulated the expression levels of TAFAZZIN, Transglutaminase 2, phosphorylated‑AKT, N‑cadherin, vimentin and proliferating cell nuclear antigen, and significantly increased those of E‑cadherin, cleaved c...
Source: Molecular Medicine - Category: Molecular Biology Authors: Source Type: research