Regulating Fibrocartilage Stem Cells via TNF- α/Nf-κB in TMJ Osteoarthritis

In this study, we investigate harnessing fibrocartilage stem cell (FCSC) capacities by regulating tumor necrosis factor α (TNF-α) signaling for cartilage repair in temporomandibular joint osteoarthritis (TMJOA). Stem cell specifics for FCSCs were characterized in the presence of TNF-α. Etanercept as a TNF-α inhibitor and BAY 11-7082 as an Nf-κB inhibitor were used to study TNF-α regulation of FCSCs. Lineage tracing was performed in Gli1-CreERT+;Tmfl/fl mice when etanercept (1 mg/kg, every 3 d) or isometric vehicle was subcutaneously injected to trace specific changes in FCSCs. Surgically induced TMJOA Sprague-Dawley rats were generated with BAY 11-7082 (5 mg/kg, every 3 d) or vehicle subcutaneous injection to investigate the functional role of TNF-α/Nf-κB in TMJOA. Anterior disc displacement (ADD) rabbits were used to analyze the therapeutic effect of etanercept as a TMJOA intra-articular treatment with etanercept (0.02 mg in 100 μL, every 2 wk) or isometric vehicle. In vitro, TNF-α inhibited proliferation of FCSCs and increased FCSC apoptosis. TNF-α activation interfered with osteogenic and chondrogenic differentiation of FCSCs, while etanercept could partially recover FCSC specificity from TNF-α. FCSC lineage tracing in Gli1-CreERT+;Tmfl/fl mice showed that the chondrogenic capacity of Gli1+ cell lineage was markedly suppressed in osteoarthritis cartilage, the phenotype of which could be significantly resc...
Source: Cell Research - Category: Cytology Authors: Source Type: research

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Progressive degenerative diseases that affect the TMJ, such as osteoarthritis (OA), juvenile idiopathic arthritis (JIA), and idiopathic condylar resorption (ICR), are characterized by ongoing loss of articular fibrocartilage, subchondral bone remodeling, and synovitis.1 These structures can be preserved by reducing inflammation and thereby slowing the progressive catabolic cellular processes underlying the pathogenesis of these conditions.1 Given that mesenchymal stem cells and their derivatives have now been employed in the treatment of numerous systemic pro-inflammatory conditions with promising outcomes, the goal of thi...
Source: Journal of Oral and Maxillofacial Surgery - Category: ENT & OMF Authors: Source Type: research
AbstractOsteoarthritis (OA) is a debilitating disease. A number of treatments like conservative approach, minimally invasive procedures and surgical therapy have proved to be beneficial. However, there is no consensus regarding the use of a single modality of treatment. Our current study evaluates the role of intra articular injection iPRF along with arthrocentesis for the management of temporomandibular joint osteoarthritis (TMJ-OA). A total of 130 patients were selected and divided into two groups, Group A (arthrocentesis only group) and Group B (arthrocentesis +i-PRF). Patients were evaluated for pain scores and maximal...
Source: Oral Surgery - Category: ENT & OMF Authors: Tags: ORIGINAL ARTICLE Source Type: research
To observe and analyze the interaction between excessive mechanical stress (MS) and decreased sex hormones on Temporomandibular Joint Osteoarthritis (TMJ-OA), and to discover TMJ-OA disease susceptibility genes by molecular biological analysis to elucidate part of the mechanism of TMJ-OA onset.
Source: Osteoarthritis and Cartilage - Category: Rheumatology Authors: Source Type: research
CONCLUSIONS: While the HCA increased in the presence of TMJ OA, no relationship was found between HCA and three different condylar positions.PMID:34355786 | DOI:10.5603/FM.a2021.0075
Source: Folia Morphologica - Category: Anatomy Authors: Source Type: research
To assess the level of evidence for genetic, biologic, and functional predictive and predisposing factors for end-stage temporomandibular joint arthritis within published literature.
Source: The British Journal of Oral and Maxillofacial Surgery - Category: ENT & OMF Authors: Tags: Review Source Type: research
Biomed Res Int. 2021 Jul 9;2021:9978651. doi: 10.1155/2021/9978651. eCollection 2021.ABSTRACTTemporomandibular joint osteoarthritis (TMJOA) is characterized by chronic inflammatory degradation of mandibular condylar cartilage (MCC). Studies have found a positive correlation between inflammation and cyclooxygenase- (COX-) 2 in OA pathology. NF-κB is a crucial transcription factor of inflammatory and immune responses in the cause of TMJOA pathology. Resveratrol (RES) plays a critical role in antioxidation and anti-inflammation. But, studies on the effects of RES on TMJOA are very limited. So, the purpose of this study ...
Source: Biomed Res - Category: Research Authors: Source Type: research
In terms of diagnostic and therapeutic management, clinicians should adequately address the frequent aspects of temporomandibular joint (TMJ) osteoarthritis (OA) associated with disk displacement. Condylar ero...
Source: BMC Oral Health - Category: Dentistry Authors: Tags: Research Source Type: research
In conclusion, in our prospective community-based study, aging-related biomarkers were associated with measures of subclinical atherosclerosis cross-sectionally and with all-cause mortality prospectively, supporting the concept that these biomarkers may reflect the aging process in community-dwelling adults. The Role of Aging Macrophages in Skin Inflammation https://www.fightaging.org/archives/2021/06/the-role-of-aging-macrophages-in-skin-inflammation/ The immune system is complex and ages in complex ways, pressed by the lifetime burden of infection and rising levels of molecular damage that trigger many...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Senescent cell accumulation appears to be a major player in the pathology of most of the joint-related issues that occur in older individuals. Senescent cells secrete signals that provoke a state of chronic inflammation, alter nearby cell behavior, and disrupt tissue structure and maintenance. Clearance of these cells reverses numerous age-related conditions and measures of aging in mice. Hence the advent of senolytic therapies that selectively destroy senescent cells is a much anticipated development in medicine. Indeed, the first such therapies are pre-existing drugs, such as the dasatinib and quercetin combination, are ...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
To explore the suppressing impact of low phosphorus intake on osteoarthritic temporomandibular joint and the possible mechanisms of nuclear acid injury in the insulted chondrocytes.
Source: Osteoarthritis and Cartilage - Category: Rheumatology Authors: Source Type: research
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