Preparation and characterization of sulfated inulin-type fructans from Jerusalem artichoke tubers and their antitumor activity

In this study, a pure inulin-type fructan, denoted as Jerusalem artichoke polysaccharide (P-JAP), was purified from Jerusalem artichoke tubers and modified by sulfation via treatment with a sulfur trioxide-pyridine complex to produce its sulfated derivative (S-JAP). Fourier-transform infrared spectroscopic analysis confirmed the successful introduction of sulfate groups. The inhibitory effects of S-JAP on the proliferation of human liver hepatocellular carcinoma (HepG2) cells was evaluated via a CCK-8 assay, and the pro-apoptotic effects were assessed using annexin V-FITC/PI double staining. The inhibition rates of various concentrations of S-JAP on HepG2 cells after 24, 48, and 72 h were significantly higher than those of P-JAP; moreover, S-JAP succeeded in promoting cell apoptosis. Thus, the sulfate-modified polysaccharide extracted from Jerusalem artichoke tubers was shown to exhibit effective antitumor activity with potential for further development.PMID:34478936 | DOI:10.1016/j.carres.2021.108422

Source: Carbohydrate Research - September 3, 2021 Category: Genetics & Stem Cells Authors: Taili Shao Pingchuan Yuan Wenzhi Zhang Deyu Dou Fengge Wang Chengyi Hao Chunyan Liu Jun Han Kaoshan Chen Guodong Wang Source Type: research