O-231 In vitro maturation of human immature (GV) oocytes after controlled ovarian hormonal stimulation with recombinant AMH in the maturation medium

AbstractStudy questionDoes the addition of recombinant AMH to thein vitro maturation (IVM) medium improve the maturation of GV oocytes after controlled ovarian hormonal stimulation?Summary answerOur results show that the addition of recombinant AMH to thein vitro maturation medium improves the maturation rate of GV oocytes.What is known alreadyAnti-M üllerian hormone (AMH) is an important hormone involved in the process of sex differentiation during embryonic development. At the transition to the 21. century, more and more researchers have studied the role of AMH in ovarian function, especially its impact on folliculogenesis. AMH is becoming on e of the main biomarkers of ovarian reserve and ovarian-specific disease, however, little is known about its effect on human oocyte maturation. Therefore, we matured immature GV (germinal vesicle) oocytes in IVM medium with recombinant AMH to assess its effect compared to the conventional IVM proced ure with FSH and hCG.Study design, size, durationIn this two-year prospective study, we compared the maturation rate of four groups of immature (GV) oocytes matured in maturation medium with added i) AMH (n  = 15), ii) AMH+FSH+hCG (n = 44), iii) FSH+hCG (conventional; n = 22), and iv) hormone-free maturation medium (control; n = 15). Each oocyte was maturedin vitro for a maximum of 28  hours and monitored by time-lapse microscopy to assess the time of GV breakdown (MI) and extrusion of the polar body (MII).Participants/ma...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research