GSE173983 RECK isoforms are differentially expressed in patients with stable and unstable coronary artery disease: A pilot study.

Contributors : Chiara Vancheri ; Elena Morini ; Francesco Romeo ; Giuseppe Novelli ; Francesca AmatiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensFour male patients were enrolled for this study in collaboration with the Cardiology Unit of Policlinico Tor Vergata-Fondazione PTV (Rome). We have performed RNA-Sequencing using NextSeq 500 ILLUMINA platform on PBMCs of patients with clinically proven healthy coronary arteries (CTR) and patients with chronic coronary artery disease (CAD) confirmed by coronary angiography. RNA sequencing results showed differentially Altenative Splicing (AS) events and filtering for a statistically significant Splicing-Index Fold-Change ≥ ±1.5 (p≤0.05) we observed 113 differentially regulated AS events (24 up and 89 down-regulated) from 86 genes. We validated AS results using Real-time PCR method and then we extended the expression analysis to a large case study including 24 CTR subjects, 72 CAD patients and 32 patients with u nstable coronary artery disease, arrived at the attention of the clinicians during a myocardial infarction event (AMI group).
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research