pattern of Candida Biofilm on Intrauterine Devices (IUDs).

pattern of Candida Biofilm on Intrauterine Devices (IUDs). J Med Microbiol. 2015 Feb 13; Authors: Zahran K, Agban MN, Ahmed SH, Hassan EA, Sabet MA Abstract Biofilms (BF) are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on removed IUDs, to perform experimental biofilm formation by isolated strains and to examine it by the crystal violet and the XTT reduction assay and scanning electron microscopy (SEM). 56 IUDs were examined for biofilm formation using Sabouraud's dextrose chloramphenicol agar. Identification of suspected colonies by different methods. Antifungal susceptibility to fluconazole and amphotericin B to isolated strains and invitro experimental biofilm formation was done. The biofilm was quantified by crystal violet, XTT reduction assay and SEM. Among the 56 IUDs investigated, 26 were Candida positive (46.4%). Candida albicans was recovered from 15 isolates. Biofilm MIC to fluconazole increased 64 to 1000 times compare to planktonic cells. XTT method was dependant on candida species; Biofilm formation was highest in Candida krusei and Candida glabrata strains followed by Candida albicans and Candida tropicalis respectively. SEM of Candida biofilm revealed a heterogeneou...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research