Optimization of a cellular HBV infection model for use in high-throughput drug screening

In this study, we optimized an in vitro HBV infection model consisting of two cell lines: HepAD38 cells, which are able to produce infectious HBV; and HepG2-NTCP cells, which are susceptible to HBV infection. We showed that prolonged production of HBV in the "donor" cells and HBV inoculation of the "acceptor" cells simultaneously with seeding improves the established procedure. This modified protocol was proven effective in experiments involving compounds with known activity against HBV, suggesting its utility for future high-throughput screening. Keywords: HBV; HBV in vitro models; HepG2-NTCP; HepAD38.PMID:33827225 | DOI:10.4149/av_2021_110

Source: Acta Virologica - April 8, 2021 Category: Infectious Diseases Authors: Nadezhda P Kartashova Svetlana I Iarovenko Ekaterina A Glubokova Ilya A Potemkin Anastasia A Karlsen Irina A Leneva Karen K Kyuregyan Alexey A Ryakhovskiy Mikhail I Mikhailov Source Type: research