Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats

Brain Res Bull. 2021 Feb 26:S0361-9230(21)00058-7. doi: 10.1016/j.brainresbull.2021.02.016. Online ahead of print.ABSTRACTThe deacetylase SIRT1 has been reported to play a critical role in regulating neurogenesis, which may be an adaptive processes contributing to recovery after stroke. Our previous work showed that the antioxidant capacity of Momordica charantia polysaccharides (MCPs) could protect against cerebral ischemia/reperfusion (I/R) after stroke. However, whether the protective effect of MCPs on I/R injury is related to NSCs proliferation remains unclear. In the present study, we designed in vivo and in vitro experiments to elucidate the underlying mechanisms by which MCPs promote endogenous NSCs proliferation during cerebral I/R. In vivo results showed that MCPs rescued the memory and learning abilities of rats after I/R damage and enhanced NSCs proliferation in the rat subventricular zone (SVZ) and subgrannular zone (SGZ) during I/R. In vitro experiments demonstrated that MCPs could stimulate the proliferation of C17.2 cells under oxygen-glucose deprivation (OGD) conditions. Further studies revealed that the proliferation-promoting mechanism of MCPs relied on increasing the activity of SIRT1, decreasing the level of deacetylation of β-catenin in the cytoplasm, and then triggering the translocation of β-catenin into the nucleus. These data provide experimental evidence that the up-regulation of SIRT1 activity by MCP led to an increased cytoplasmic deacetylation o...
Source: Brain Research Bulletin - Category: Neurology Authors: Source Type: research