LncRNA ZFAS1 inhibits triple-negative breast cancer by targeting STAT3.

LncRNA ZFAS1 inhibits triple-negative breast cancer by targeting STAT3. Biochimie. 2021 Jan 08;: Authors: Sharma U, Barwal TS, Khandelwal A, Malhotra A, Rana MK, Singh Rana AP, Imyanitov EN, Vasquez KM, Jain A Abstract Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer with fewer treatment options than other types of invasive breast cancer due to the loss of the estrogen, progesterone receptors and low levels of the HER2 protein, resulting in a poor prognosis for these patients. Here, we found that the expression of the lncRNA, ZFAS1, was significantly downregulated (∼3.0-fold) in blood samples of TNBC patients (n = 40) compared to matched healthy controls (n = 40). Functionally, silencing of ZFAS1 promoted cell proliferation and colonization of human MDA-MB-231 TNBC cells by inhibiting the expression levels of the cyclin-dependent kinase (CDK) inhibitors p21 (CDKN1A) and p27 (CDKN1B) compared to the scrambled siRNA control cells. Further, we found that downregulation of ZFAS1 led to decreased protein levels of the epithelial markers, E-cadherin, Claudin-1, and Zo-1, with increased protein levels of the mesenchymal markers, Slug and ZEB1. In addition, by utilizing the bioinformatic tools such as RAID v2.0 (RNA Interactome Database Version 2.0), AnnoLnc (Annotate human lncRNA database), and GEPIA (Gene Expression Profiling Interactive Analysis), we identified a strong negative correlation be...
Source: Biochimie - Category: Biochemistry Authors: Tags: Biochimie Source Type: research