Vaccination with chimeric protein induces protection in murine model against ascariasis.

Vaccination with chimeric protein induces protection in murine model against ascariasis. Vaccine. 2020 Nov 26;: Authors: de Castro JC, de Almeida LV, Cardoso MS, Oliveira FMS, Nogueira DS, Reis-Cunha JL, Magalhaes LMD, Zhan B, Bottazzi ME, Hotez PJ, Bueno LL, Bartholomeu DC, Fujiwara RT Abstract An estimated 400 million people are infected by parasites of the genus Ascaris and the existing control measures are inefficient. Vaccine development using B cell antigens is a promising strategy for increased protection against this parasite. The present study aimed at developing a chimeric protein capable of conferring protection against infection by Ascaris sp. For this purpose, we performed B-cell epitope predictions on previously described vaccine candidate proteins from Ascaris suum and the corresponding peptides were used to construct a chimeric protein. Female BALB / c mice were immunized subcutaneously in three doses at 10 day intervals with a vaccine formulation comprised of the chimeric protein together with monophosphoryl lipid A (MPLA). Control groups included protein alone, MPLA, or PBS. After challenge infection, animals vaccinated with chimeric protein plus MPLA showed a reduction of 73.54% of larval load in the lung compared to control group animals. Animals immunized with chimeric protein plus MPLA also display higher IgG response and a reduction in lung inflammation. Our study highlights how chimeric proteins containing mo...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research