The effect of combined curcumin-mediated photodynamic therapy and artificial skin on Staphylococcus aureus –infected wounds in rats

This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with artificial skin on disinfection of infected skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n = 6): (i) control —untreated; (ii) aPDT—treated with curcumin-mediated aPDT (blue light); (iii) artificial skin—treated with artificial skin alcohol-based; and (iv) aPDT plus artificial skin—treated with aPDT associated with artificial skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0. 80 cm was performed in order to inoculate the microorganismStaphylococcus aureus (ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm2) at the dose of 60 J/cm2 and the treatment with alcohol-based artificial skin was done with the topical application of 250 μL. Additional animals were submitted to aPDT combined with the artificial skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability wa s observed in the PDT plus artificial skin group (4.14 log10), followed by artificial skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for artificial skin, and 35.02% for PDT plus artificial) i n comparison with the control group. These findings pro...
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research