Digital PCR for the Analysis of MYC Copy Number Variation in Lung Cancer.

Digital PCR for the Analysis of MYC Copy Number Variation in Lung Cancer. Dis Markers. 2020;2020:4176376 Authors: Brik A, Weber DG, Casjens S, Rozynek P, Meier S, Behrens T, Stamatis G, Darwiche K, Theegarten D, Brüning T, Johnen G Abstract Background: MYC (v-myc avian myelocytomatosis viral oncogene homolog) is one of the most frequently amplified genes in lung tumors. For the analysis of gene copy number variations, dPCR (digital PCR) is an appropriate tool. The aim of our study was the assessment of dPCR for the detection of MYC copy number variations (CNV) in lung tissue considering clinicopathological parameters. Material and Methods. MYC status was analyzed with dPCR as well as qPCR (quantitative PCR) using gDNA (genomic DNA) from tumor and adjacent nontumor tissue samples of lung cancer patients. The performance of MYC was estimated based on the AUC (area under curve). Results: The results of the MYC amplification correlated significantly between dPCR and qPCR (r S = 0.81, P
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research

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Conclusion: 5-HMF treatment decreased P50, improved SaO2, and mitigated increases in PAP in this swine model of NH. Introduction The reduction of barometric pressure at altitude is associated with reduced partial pressure of ambient Oxygen (PO2). With lower ambient PO2, it can be anticipated that alveolar (PAO2), arterial O2 (PaO2) and blood O2 content (CaO2) will decrease accordingly, resulting in a widely recognized decrease in maximal O2 consumption (VO2) (Dill and Adams, 1972; Lucas et al., 2011). In humans, with acute exposure to altitude, maximal VO2 decrements are measurable at elevations even as low as 580 ...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
Markus Hartl* and Rainer Schneider Center of Molecular Biosciences (CMBI), Institute of Biochemistry, University of Innsbruck, Innsbruck, Austria The neuronal proteins GAP43 (neuromodulin), MARCKS, and BASP1 are highly expressed in the growth cones of nerve cells where they are involved in signal transmission and cytoskeleton organization. Although their primary structures are unrelated, these signaling proteins share several structural properties like fatty acid modification, and the presence of cationic effector domains. GAP43, MARCKS, and BASP1 bind to cell membrane phospholipids, a process reversibly regulate...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Swetha Rajasekaran1,2,3†, Lakshmi Dhevi Nagarajha Selvan4†, Kathleen Dotts1,2,3†, Ranjith Kumar4, Pukhraj Rishi5, Vikas Khetan5, Madhoolika Bisht1,2,3, Karthikeyan Sivaraman6, Subrmanian Krishnakumar7, Debashis Sahoo8, Moray J. Campbell9, Sailaja V. Elchuri4* and Wayne O. Miles1,2,3* 1Department of Molecular Genetics, The Ohio State University, Columbus, OH, United States2The Ohio State University Comprehensive Cancer Center, Columbus, OH, United States3Center for RNA Biology, The Ohio State University, Columbus, OH, United States4Department of Nanobiotechnology, Vision Research Foundation, Sank...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Monica Parodi1, Herman Favoreel2, Giovanni Candiano3, Silvia Gaggero4, Simona Sivori4,5, Maria Cristina Mingari1,4,5, Lorenzo Moretta6, Massimo Vitale1 and Claudia Cantoni4,5,7* 1Immunology Operative Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy 2Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium 3Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy 4Department of Experimental Medicine, University of Genoa, Genoa, Italy 5Center of Excellence for Biomedical Research, University of Genoa, Genoa, Italy ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
AbstractWe previously reported on a family with hereditary lung cancer, in which a germline mutation in the transmembrane domain (G660D) of avian erythroblastic leukemia viral oncogene homolog 2 (erb‐b2 receptor tyrosine kinase 2) (ERBB2; human epidermal growth factor receptor 2 [HER2]) seemed to be responsible for the cancer predisposition. Although few data are available on treatment, anti‐ERBB2 therapeutic agents may be effective for ERBB2‐mutant cancers. The familial lung cancer patient in one of the authors’ institutes developed bone metastasis with enlarging lung tumors and was treated with the ERBB2 inhi...
Source: The Oncologist - Category: Cancer & Oncology Authors: Tags: Precision Medicine, Precision Medicine Clinic: Molecular Tumor Board Source Type: research
Abstract We previously reported on a family with hereditary lung cancer, in which a germline mutation in the transmembrane domain (G660D) of avian erythroblastic leukemia viral oncogene homolog 2 (erb-b2 receptor tyrosine kinase 2) (ERBB2; human epidermal growth factor receptor 2 [HER2]) seemed to be responsible for the cancer predisposition. Although few data are available on treatment, anti-ERBB2 therapeutic agents may be effective for ERBB2-mutant cancers. The familial lung cancer patient in one of the authors' institutes developed bone metastasis with enlarging lung tumors and was treated with the ERBB2 inhibi...
Source: The Oncologist - Category: Cancer & Oncology Authors: Tags: Oncologist Source Type: research
Authors: Bian Q, Lu J, Zhang L, Chi Y, Li Y, Guo H Abstract Outbreaks of avian influenza A (H5N1) virus infection have significant health and economic consequences. Non-structural protein 1 (NS1) is an essential virulence factor of the highly pathogenic H5N1 avian influenza virus and of the apoptosis associated with the pathogenesis of H5N1. Previous studies have revealed that the NS1 protein is able to induce apoptosis via an extrinsic pathway. However, it remains unclear whether the intrinsic pathway is also associated with this apoptosis. The present study used a clone of the NS1 gene from avian influenza A/Jian...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
AbstractHuman lung cancer cells (Calu-3 line) were studied for the development of apoptosis, necrosis, and autophagy in response to infection with orthoand paramyxoviruses. Biochemical pathways underlying various mechanisms of cell death differed for different viruses. When infected with murine Sendai paramyxovirus, Calu-3 cells demonstrated typical necrotic features such as cell swelling (but not shrinkage), lack of chromatin DNA laddering, of caspase 3 and 8 activation, and of apoptotic cleavage of poly(ADP-ribose) polymerase (PARP) protein; an activation of antiapoptotic protein kinase Akt was also revealed. In contrast...
Source: Biochemistry (Moscow) - Category: Biochemistry Source Type: research
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Source: Molecular Carcinogenesis - Category: Molecular Biology Authors: Tags: RESEARCH ARTICLE Source Type: research
Lung cancer is among the leading causes of cancer-related deaths, and thusmonitoring, treatment and course of the disease has become a major area of research following diagnosis of the disease. The present study aimed to evaluate the role of HIF pathway in development of non-small-cell lung cancer (NSCLC) in patients diagnosed with NSCLC using their tumor and non-tumor tissue specimens.Both tumor and non-tumor tissue specimens were collected from 10 patients with NSCLC.They were placed in Trizol, and stored at - 80 C ° until processing. RNA was isolated from these tissue specimens using the Trizol/chloroform method. Th...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: 11.1 Lung Cancer Source Type: research
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