Utility of the Rose Bengal Test as a Point-of-Care Test for Human Brucellosis in Endemic African Settings: A Systematic Review.

Utility of the Rose Bengal Test as a Point-of-Care Test for Human Brucellosis in Endemic African Settings: A Systematic Review. J Trop Med. 2020;2020:6586182 Authors: Ekiri AB, Kilonzo C, Bird BH, VanWormer E, Wolking DJ, Smith WA, Masanja H, Kazwala RR, Mazet JAK Abstract In endemic African areas, such as Tanzania, Brucella spp. cause human febrile illnesses, which often go unrecognized and misdiagnosed, resulting in delayed diagnosis, underdiagnosis, and underreporting. Although rapid and affordable point-of-care tests, such as the Rose Bengal test (RBT), are available, acceptance and adoption of these tests at the national level are hindered by a lack of local diagnostic performance data. To address this need, evidence on the diagnostic performance of RBT as a human brucellosis point-of-care test was reviewed. The review was initially focused on studies conducted in Tanzania but was later extended to worldwide because few relevant studies from Tanzania were identified. Databases including Web of Science, Embase, MEDLINE, and World Health Organization Global Index Medicus were searched for studies assessing the diagnostic performance of RBT (sensitivity and specificity) for detection of human brucellosis, in comparison to the reference standard culture. Sixteen eligible studies were identified and reviewed following screening. The diagnostic sensitivity (DSe) and specificity (DSp) of RBT compared to culture as the gold standard were 87.5% and 100%, resp...
Source: Journal of Tropical Medicine - Category: Tropical Medicine Tags: J Trop Med Source Type: research

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Neuropediatrics DOI: 10.1055/s-0040-1715482Brucellosis is one of the most common zoonosis worldwide. It is still endemic in many regions of the world. A 6-year-old female was admitted to the emergency department (ED) due to a sudden change in consciousness, urinary incontinence, vomiting, and difficulty in walking. Neurological examination demonstrated abducens nerve paralysis, mild-to-moderate motor deficit in hemiparesis in the left arm. Brain magnetic resonance imaging showed a hemorrhagic focus at the right frontal lobe and thrombosis in the superior sagittal sinus of the brain. The diagnosis of neurobrucellosis was co...
Source: Neuropediatrics - Category: Neurology Authors: Tags: Short Communication Source Type: research
Brucellosis is a zoonotic disease caused by a fastidious Gram-negative bacteria. Most commonly, it is transmitted from the consumption of unpasteurized dairy products and rarely from human to human. Endemic areas include Africa, the Mediterranean Basin, the Middle East, and Central and South Americas. Symptoms are often nonspecific including fever, fatigue, and decreased appetite. Brucella poses a real danger to lab personnel if not notified by the suspecting physician. Both the health of the patient and laboratory staff is at risk1.
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Clinical Communication to the Editor Source Type: research
CONCLUSION: It should be kept in mind that brucellosis may occur in patients presenting with fever, sweating, arthralgia and leukopenia, especially in endemic areas where dairy livestock is prevalent. People living in these areas should be educated about proper animal care and the handling of dairy products. PMID: 33000109 [PubMed - in process]
Source: Ann Acad Med Singapo... - Category: General Medicine Authors: Tags: Ann Acad Med Singap Source Type: research
In this study, the epidemiological characteristics of human brucellosis were analyzed, and the genotypic profile and antimicrobial susceptibilities ofBrucella melitensis strains isolated from humans in Hinggan League were investigated.MethodsThe epidemic characteristics were described using case number, constituent ratio, and rate. The 418 human blood samples were collected and tested by bacteriology, and suspect colonies were isolated and identified by conventional biotyping assays, the VITEK 2.0 microbial identification system, and AMOS (Brucella abortus,B. melitensis,B. ovis, andB. suis)-PCR. Subsequently, all strains w...
Source: Infectious Diseases of Poverty - Category: Infectious Diseases Source Type: research
Abstract Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture methods in order to detect Brucella melitensis in raw milk samples of 55 sheep and 45 goats through deriving the primers from the omp31 element of the Brucella genome. In addition, nine isolates of B. melitensis were identified using the culture method. No positive cases were found in sediment s...
Source: Animal Biotechnology - Category: Biotechnology Authors: Tags: Anim Biotechnol Source Type: research
Abstract Brucellosis is the most common zoonosis in the world. It can affect several organs or systems, of which the genitourinary is the second most common after the musculoskeletal. Renal involvement in brucellosis takes the form of IgA nephropathy, interstitial nephritis, pyelonephritis, mixed cryoglobulinemia and kidney failure. While the agent can be isolated in urine, renal involvement is rare (
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
by Ramin Bagheri Nejad, Rosina C. Krecek, Omar H. Khalaf, Nabil Hailat, Angela M. Arenas-Gamboa Brucellosis is a bacterial endemic zoonotic disease of global significance with detrimental impacts on public health and food animal production. It is caused byBrucella spp., an expanding group of pathogens able to infect various host species. Bovines and small ruminants, which excrete the bacteria in milk and in reproductive discharges, are major sources of infection for humans and other animals. Contact with contaminated animals and consumption of unpasteurized dairy products are the main routes for human infection. In spite ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
CONCLUSIONS: Osteoarticular involvement was detected in nearly one of every four childhood brucellosis patients in our study. Brucellosis should be considered as a pre-diagnosis in children with osteoarticular complaints, especially in regions where the disease is endemic. PMID: 32419411 [PubMed - in process]
Source: The Turkish Journal of Pediatrics - Category: Pediatrics Authors: Tags: Turk J Pediatr Source Type: research
Abstract Iran which is regarded as an endemic region for brucellosis ranked second in brucella prevalence in the world. Pulmonary involvement is a rare complication of brucellosis. In this article, we aimed to report a case of systemic brucellosis complicated with brucella pneumonia. A 39-yearsold man was referred to the emergency department with weakness, productive coughs and severe weight loss during 8 months. Agglutination tests for brucellosis showed high titers suggestive for brucella infection. After 6 days of treatment, the patient clinical state has improved significantly. The patient had systemic signs a...
Source: Infectious Disorders Drug Targets - Category: Infectious Diseases Authors: Tags: Infect Disord Drug Targets Source Type: research
ary U, Wensman JJ Abstract An extended range of host susceptibility including camel has been evidenced for some of the important veterinary and public health pathogens, such as brucellosis, peste des petits ruminants (PPR) and bluetongue (BT). However, in disease endemic settings across many parts of the globe, most of the disease control interventions accounts for small and large ruminants, whereas unusual hosts and/or natural reservoirs, such as camels, remain neglected for disease control measures including routine vaccination. Such a policy drawback not only plays an important role in disease epizootiology par...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
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