Molecular detection of Theileria annulata infection in cattle by conventional PCR and quantitative real time PCR in India

The objective of this study is to identifyT. annulata infection in the peripheral blood of cattle as a biological sample by conventional PCR (cPCR) and quantitative PCR (qPCR). The phylogenetic analysis was done using theT. annulata merozoite surface antigen (Tams 1) gene. Out of 552 samples of examined blood smears by microscopy, 454 (82.24%) animals were positive forTheileria species. Out of 454 samples, 96 samples were further examined by both cPCR and qPCR, 52 samples (54.16%) were found positive forT. annulata in both PCR methodologies. Phylogenetic analysis revealed thatT. annulata Odisha isolate was closely related toT. annulata Uttarakhand, India isolate (KM061799) and Hyderabad, India isolate (MK034702) with Nucleotide sequence identity 95.36%, 95.25%, respectively. This is the first study to detectT. annulata by qPCR in Odisha and supported that both PCR techniques were equally effective for the detection ofTams 1 gene ofT. annulata in cattle ’s blood.
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research