Dynamics of bla KPC-2 dissemination from non-CG258 Klebsiella pneumoniae to other Enterobacterales via IncN plasmids in an area of high endemicity.

Dynamics of bla KPC-2 dissemination from non-CG258 Klebsiella pneumoniae to other Enterobacterales via IncN plasmids in an area of high endemicity. Antimicrob Agents Chemother. 2020 Sep 21;: Authors: Rada AM, De La Cadena E, Agudelo C, Capataz C, Orozco N, Pallares C, Dinh AQ, Panesso D, Ríos R, Diaz L, Correa A, Hanson BM, Villegas MV, Arias CA, Restrepo E Abstract Carbapenem-resistant Enterobacterales (CRE) pose a significant threat to global public health. The most important mechanism for carbapenem resistance is the production of carbapenemases. Klebsiella pneumoniae carbapenemase (KPC) represents one of the main carbapenemases worldwide. Complex mechanisms of bla KPC dissemination have been reported in Colombia, a country with high endemicity of carbapenem resistance. Here, we characterized the dynamics of dissemination of bla KPC gene among CRE infecting and colonizing patients in three hospitals localized in a highly endemic area of Colombia (2013 and 2015). We identified the genomic characteristics of KPC-producing Enterobacterales recovered from patients infected/colonized and reconstructed the dynamics of dissemination of bla KPC-2 using both short and long read sequencing. We found that spread of bla KPC-2 among Enterobacterales in the participating hospitals was due to intra and inter-species horizontal gene transfer (HGT) mediated by promiscuous plasmids associated to transposable elements that was originated from a mul...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research