Cancers, Vol. 12, Pages 2680: MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance

Cancers, Vol. 12, Pages 2680: MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance Cancers doi: 10.3390/cancers12092680 Authors: Milosz Wilczynski Michal Kielbik Daria Senderowska Tomasz Krawczyk Bozena Szymanska Magdalena Klink Jan Bieńkiewicz Hanna Romanowicz Filip Frühauf Andrzej Malinowski High levels of miRNA-103/107 are associated with poor outcomes in the case of breast cancer patients. MiRNA-103/107-DICER axis may be one of the key regulators of cancer aggressiveness. MiRNA-103/107 expression levels have never been related to patients’ clinicopathological data in epithelial ovarian cancer. We aimed to assess miRNA-103/107 expression levels in high grade serous ovarian cancer tissues. Expression levels of both miRNAs were related to the clinicopathological features and survival. We also evaluated expression levels of miRNA-103/107 and DICER in selected ovarian cancer cell lines (A2780, A2780cis, SK-OV-3, OVCAR3). We assessed the relative expression of miRNA-103/107 (quantitative reverse transcription-polymerase chain reaction) in fifty archival formalin-fixed paraffin-embedded tissue samples of primary high grade serous ovarian cancer. Then, miRNA-103/107 and DICER expression levels were evaluated in selected ovarian cancer cell lines. Additionally, DICER, N-/E-cadherin protein levels were assessed with the use of western blot. We identified miRNA-107 up-regulation in ovarian cancer in compari...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research