In-depth quantitative proteomics uncovers specie-specific metabolic programs in < i > Leishmania (Viannia) < /i > species

by Nathalia Pinho, Jacek R. Wi śniewski, Geovane Dias-Lopes, Leonardo Saboia-Vahia, Ana Cristina Souza Bombaça, Camila Mesquita-Rodrigues, Rubem Menna-Barreto, Elisa Cupolillo, Jose Batista de Jesus, Gabriel Padrón, Patricia CuervoLeishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenicLeishmania spp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and theLeishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representingL.braziliensis,L.panamensis andL.guyanensis species. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. “Total Protein Approach” and “Proteomic Ruler” were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins , representing the most completeLeishmania proteome yet known, and provides a comprehensive quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research