In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance.

In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance.
Methods Mol Biol. 2020;2171:201-214
Authors: Vales S, Poling HM, Sundaram N, Helmrath MA, Mahe MM
Abstract
Human intestinal organoids (HIOs), derived from pluripotent stem cells, are a new tool to gain insights in gastrointestinal development, physiology, and associated diseases. Herein, we present a method for renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial cell proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific steps to successful engraftment and provides insight into the study of human intestinal stem cells.
PMID: 32705643 [PubMed - in process]

Source: Mol Biol Cell - July 28, 2020 Category: Molecular Biology Authors: Vales S, Poling HM, Sundaram N, Helmrath MA, Mahe MM Tags: Methods Mol Biol Source Type: research