Conjugation of micro/nanocurcumin particles to ZnO nanoparticles changes the surface charge and hydrodynamic size thereby enhancing its antibacterial activity against Escherichia coli and Staphylococcus aureus

Nanobiotechnology mediated synthesis of curcumin ‐ZnO nanocomposites with reduced size and improved bioactivity AbstractNanobiotechnology ‐mediated synthesis of ZnO nanoparticles, micro/nanocurcumin, and curcumin–ZnO nanocomposites and their characterization followed by comparative study of their antibacterial, antioxidant, and iron‐chelating efficiency at various dosages are discussed. Micro/nanocurcumin and ZnO nanoparticles w ere synthesized using curcumin and zinc nitrate as precursor and then conjugated by sonication to synthesize curcumin–ZnO nanocomposites. The synthesized nanoparticles were then characterized by using ultraviolet‐visible spectroscopy, X‐ray diffraction, Scanning electron microscopy, Fourier‐ transform infrared spectroscopy, and dynamic light scattering analysis. After that, the antibacterial activity of the synthesized nanoparticles was evaluated by the optical density (OD600) method againstEscherichia coli andStaphylococcus aureus cells. The DPPH (2,2 ‐diphenyl‐1‐picrylhydrazyl ), hydroxyl radical scavenging activity, and ferrous ion–chelating efficiency of synthesized nanoparticles were evaluated by spectrophotometry analysis. Nanocurcumin (mean zeta potential = −25 mV; average hydrodynamic diameter = 410 nm) based coating of ZnO nano particles (mean zeta potential = −15.9 mV; average hydrodynamic diameter = 274 nm) to synthesize curcumin–ZnO nanocomposites (mean zeta potential = −18.8 mV; average hydrodynamic diame...
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: Original Article Source Type: research