Generation of primary human intestinal T cell transcriptomes reveals differential expression at genetic risk loci for immune-mediated disease

Conclusions
This is the first report of transcriptomes for minimally manipulated intestinal T lymphocyte subsets in humans. We have demonstrated that careful processing of mucosal biopsies allows the generation of transcriptomes from as few as 1000 highly purified cells with minimal interindividual variation. Bioinformatic integration of transcriptomic data with recent GWAS data identified specific candidate genes and cell types for inflammatory pathologies.

Source: Gut - January 8, 2015 Category: Gastroenterology Authors: Raine, T., Liu, J. Z., Anderson, C. A., Parkes, M., Kaser, A. Tags: Crohn's disease, Ulcerative colitis, Open access, Editor's choice, Coeliac disease Intestinal inflammation Source Type: research