iTRAQ-based proteomic analysis after mesenchymal stem cell line transplantation for ischemic stroke.
iTRAQ-based proteomic analysis after mesenchymal stem cell line transplantation for ischemic stroke.
Brain Res. 2020 May 20;:146900
Authors: Mitaki S, Nagai A, Wada Y, Onoda K, Md Sheikh A, Adachi E, Matsumoto KI, Yamaguchi S
Abstract
Transplantation with mesenchymal stem cells (MSCs) has been reported to promote functional recovery in animal models of ischemic stroke. However, the molecular mechanisms underlying the therapeutic effects of MSC transplantation have been only partially elucidated. The purpose of this study was to comprehensively identify changes in brain proteins in rats treated with MSCs for ischemic stroke, and to explore the multi-target mechanisms of MSCs using a proteomics-based strategy. Twenty-eight proteins were found to be differentially expressed following B10 MSC transplantation in adult male Wistar rats, as assessed using isobaric tagging for relative and absolute protein quantification (iTRAQ). Subsequent bioinformatic analysis revealed that these proteins were mainly associated with energy metabolism, glutamate excitotoxicity, oxidative stress, and brain structural and functional plasticity. Immunohistochemical staining revealed decreased expression of EAAT1 in the phosphate-buffered saline group as opposed to normal levels in the B10 transplantation group. Furthermore, ATP levels were also significantly higher in the B10 transplantation group, thus supporting the iTRAQ results. Our results suggest that t...
Source: Brain Research - Category: Neurology Authors: Mitaki S, Nagai A, Wada Y, Onoda K, Md Sheikh A, Adachi E, Matsumoto KI, Yamaguchi S Tags: Brain Res Source Type: research
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