Multiple stressor responses are regulated by sirtuins in Mytilus congeners.

Multiple stressor responses are regulated by sirtuins in Mytilus congeners. Comp Biochem Physiol A Mol Integr Physiol. 2020 May 04;:110719 Authors: Vasquez MC, Martinez DA, Tomanek L Abstract Understanding physiological tolerances of marine organisms to environmental stress is key to predicting species susceptability under climate change. Along the Pacific Coast of the U.S.A. intertidal mussel congeners (genus Mytilis) vary in their physiological stress tolerances, with the invasive M. galloprovincialis being heat tolerant but vulnerable to hyposalinity while the native M. trossulus is vulnerable to heat stress and tolerant of hyposalinity. Sirtuins, a family of NAD+-dependent deacylases, may influence the environmental stressor tolerances in these mussel congeners. The purpose of our study was to determine the mechanism by which sirtuins may confer differential stress responses in the two mussel congeners. Mussels (N = 6 per species) were acclimated to laboratory conditions in tidal simulators and exposed to sirtuin inhibitors (suramin and nicotinamide). Following inhibition, mussels were exposed to hyposalinity stress (29 ppt) for 6 h followed by aerial heat stress (32 °C) for 6 h after which mussel gill was dissected for proteomic analysis. During sirtuin inhibition we found a reduction of cellular stress response (CSR) proteins (molecular chaperones, antioxidants), which are key to maintaining cellular homeostasis. M...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - Category: Physiology Authors: Tags: Comp Biochem Physiol A Mol Integr Physiol Source Type: research