GSE150078 Analysis of microRNA expression profiles and prediction of target gene signaling pathways in familial hypertrophic cardiomyopathy with myosin-binding protein C3 (MYBPC3) gene mutations

Contributors : Lihong Lu ; Xuequn Hu ; Jiazhen Dai ; Ningning Lin ; Lirong Lin ; Rehua Wang ; Zhangxin Xie ; Xuemei ChenSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensWe investigated the role of microRNA (miRNAs) in familial hypertrophic cardiomyopathy (FHCM) with MYBPC3 gene mutations. We investigated the miRNAs expression profile in three patients with FHCM with MYBPC3 gene mutations and compared the miRNA expression profiles with those of the normal control group by miRNA-seq. The significantly differentially expressed miRNAs was verified by real-time polymerase chain reaction (PCR). Target genes and signaling pathways of the identified differentially expressed miRNAs were predicted by bioinformatics analysis. The three probands were all hypertrophic obstructive cardiomyopathy with severe myocardial hypertrophy, and two of the families had a family history of sudden cardiac death, representing a “malignant” phenotype. A total of 33 significantly differentially expressed miRNAs were detected in the peripheral blood of the three probands, of which 28 were upregulated and 5 were downregulated. Real-time PCR confirmed that the expression of miR-208b-3p was consistent with the microarray res ults, and the difference was statistically significant (P＜0.05). Bioinformatics analysis showed that miR-208b-3p was mainly enriched in 79 target genes including Dally, Frizzled, Dvl, Nlk, Stbm and CaMKII, and gene ontology (GO) anal...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research