Targeted Photodynamic Therapy of Human Head and Neck Squamous Cell Carcinoma with Anti ‐epidermal Growth Factor Receptor Antibody Cetuximab and Photosensitizer IR700DX in the Mouse Skin‐fold Window Chamber Model

In this study the EGFR over‐expressing human head/neck OSC‐19‐luc2‐cGFP tumour with transfected GFP gen e was used in a skin‐fold window‐chamber model in BALB/c nude mice. The uptake and localization of the conjugate in the tumour and its surrounding normal tissues were studied by an intravital confocal laser scanning microscopy with image analyses. The tumour was also irradiated with 690 nm las er light 24 hours after conjugate administration. The vascular and tumour responses were examined by morphological evaluation and immunohistochemistry (IHC). The amount of conjugate in the tumour peaked at 24‐48 hours after injection. Image analyses of colocalization correlation parameters demonst rated a high fraction of the conjugate IR700DX colocalised in the GFP‐expressing tumour cells. PDT‐treated tumours showed extensive necrotic/apoptotic destruction with little vascular damage; while IHC showed no HIF‐1α expression and decreased EGFR and Ki67 expression with activated caspase‐ 3 overexpression, indicating a direct killing of tumour cells through both necrotic and apoptotic cell death.
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research

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This study aims to determine the impact of sarcopenia on survival and late toxicity in a large cohort of head and neck squamous cell carcinoma (HNSCC) patients treated with definitive (chemo)radiotherapy ((C)RT). MATERIALS AND METHODS: HNSCC patients treated with definitive (C)RT from January 2007 to June 2016 were included. Sarcopenia was assessed from radiation planning computed tomography (CT) scans using skeletal muscles at level C3. The impact of sarcopenia on overall survival (OS) and disease-free survival (DFS) was evaluated using the Kaplan-Meier method. Multivariable association models were developed to asses...
Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology - Category: Radiology Authors: Tags: Radiother Oncol Source Type: research
CONCLUSION: Induction chemotherapy with Cisplatin and Docetaxel is a promising regimen with good response and favorable toxicity profile and can be considered as a potentially effective outpatient regimen for locally advanced squamous cell carcinoma of head and neck. PMID: 32212813 [PubMed - in process]
Source: Asian Pacific Journal of Cancer Prevention - Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research
We previously reported outcomes for de-escalated chemoRT in HPV+ OPC by assessing tumor hypoxia with pre and intra-treatment (rx) dynamic 18F-FMISO (fluoromisonidazole) PET. Patients with absence of hypoxia on pre- or intra-rx scan were de-escalated to 30Gy, whereas those who remained hypoxic received 70Gy. Here, we sought to define genetic and/or micro-environmental factors that may influence clinical outcomes to de-escalated therapy.
Source: International Journal of Radiation Oncology * Biology * Physics - Category: Radiology Authors: Tags: Definitive Management of Head and Neck Squamous Cell Carcinoma Source Type: research
Positron emission tomography/contrast-enhanced computed tomography (PET/CECT) scan is an important tool for monitoring treatment response in head and neck cancer. For locally advanced oral squamous cell carcinoma (OSCC) treated by surgery and adjuvant therapy, consensus has yet to be reached on whether the optimal time to initiate surveillance PET/CECT is before or after adjuvant therapy. We sought to assess the utility of PET/CECT scans obtained 3 months after adjuvant therapy for locally advanced OSCC.
Source: International Journal of Radiation Oncology * Biology * Physics - Category: Radiology Authors: Tags: Management of Recurrent/Metastatic Head and Neck Squamous Cell Carcinoma Source Type: research
AbstractCancer progression can be understood as the result of deregulation of tumors ’ immune microenvironments. Recent studies of the alterations of microenvironments highlight their significant influence on the prognosis of patients with head and neck squamous cell carcinoma (HNSCC). It is necessary to better characterize tumor-infiltrating lymphocytes by focusing, in particular , on the tumor escape mechanisms from immune surveillance. One of the best described tumor immune system evasion mechanisms is the expression of co-stimulation molecules that constitute so-called “immune checkpoints”. These mole...
Source: Head and Neck Pathology - Category: Pathology Source Type: research
Discussion and Review of the Literature. Oncologist. 2020 Feb 24;: Authors: Grecea M, Marabelle A, Ammari S, Massard C, Champiat S Abstract A breakthrough in oncology over the last 5 years, immunotherapy has proved its salutary effects in a wide range of solid tumors. The targeting of the programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) pathway can restore a competent antitumor T-cell response by addressing key tumor immune evasion mechanisms. This novel mechanism of action is associated with new patterns of responses that were not observed with conventional treatments s...
Source: The Oncologist - Category: Cancer & Oncology Authors: Tags: Oncologist Source Type: research
In this study, we examined the relationships between systemic inflammatory parameters and FDG-PET/CT parameters in advanced head and neck squamous cell carcinoma (HNSCC).
Source: Auris, Nasus, Larynx - Category: ENT & OMF Authors: Tags: Original Article Source Type: research
AbstractPurposeThe linkage between the genetic and phenotypic heterogeneity of the tumor has not been thoroughly evaluated. Herein, we investigated how the genetic and metabolic heterogeneity features of the tumor are associated with each other in head and neck squamous cell carcinoma (HNSC). We further assessed the prognostic significance of those features.MethodsThe mutant-allele tumor heterogeneity (MATH) score (n = 508), a genetic heterogeneity feature, and tumor glycolysis feature (GlycoS) (n = 503) were obtained from the HNSC dataset in the cancer genome atlas (TCGA). We identified matchin...
Source: EJNMMI Research - Category: Radiology Source Type: research
In conclusion, a novel yet reliable method for the measurement of intracellular 18F-FDG via GS has been developed, and our results indicate that 18F-FDG uptake is associated with radiation and cetuximab response in HNSCC. PMID: 31620196 [PubMed]
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
This study aims to investigate the changes of hypoxia measured by 18F-fluoroerythronitroimidazole (FETNIM) uptake on integrated positron emission tomography and computed tomography (PET/CT) during chemoradiotherapy and its prognostic value of clinical outcome in locoregionally advanced HNSCC. Thirty-two patients with locoregionally advanced HNSCC who received definitive treatment with concurrent chemoradiotherapy underwent FETNIM PET/CT scans before and after 5 weeks of treatment. The intensity of hypoxia using the maximum standardized uptake value (SUVmax) was evaluated both on primary lesion and metastatic lymph node (M...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
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