Oxidation of Parathyroid Hormone.

Oxidation of Parathyroid Hormone. Clin Chim Acta. 2020 Mar 13;: Authors: Ursem SR, Vervloet MG, de Jongh RT, Heijboer AC Abstract Parathyroid hormone (PTH) is the key hormone regulating calcium homeostasis and, as such, is an important diagnostic and prognostic marker. Although the measurement of PTH has greatly improved over the past few decades, oxidation status thereof is unaccounted for in currently used assays. For example, PTH can be oxidized on methionine residues located at amino acid positions 8 and 18. This post-translational modification is relevant because it results in PTH refolding and decreased activation of the PTH1 receptor. Although loss of activity after oxidation was observed as early as 1934, only recently a method was developed to measure and distinguish non-oxidized PTH (n-oxPTH) from oxidized PTH thus improving our understanding of their roles in physiology and pathophysiology. Herein, we review the available evidence of the effect of oxidation on the biological activity of PTH. We also discuss studies examining the mechanism of PTH oxidation in vivo and efforts to use synthetic PTH ex vivo for therapeutic applications. Lastly, the available studies regarding the clinical significance of n-oxPTH are evaluated and future directions discussed. PMID: 32178977 [PubMed - as supplied by publisher]
Source: International Journal of Clinical Chemistry - Category: Chemistry Authors: Tags: Clin Chim Acta Source Type: research