Development of mismatch amplification mutation assay (MAMA) for the rapid differentiation of Mycoplasma gallisepticum K vaccine strain from field isolates.

Development of mismatch amplification mutation assay (MAMA) for the rapid differentiation of Mycoplasma gallisepticum K vaccine strain from field isolates. Avian Pathol. 2020 Mar 17;:1-24 Authors: Bekő K, Kovács ÁB, Kreizinger Z, Marton S, Bányai K, Bánáti L, Catania S, Bradbury J, Lysnyansky I, Olaogun OM, Gyuranecz M Abstract Mycoplasma gallisepticum causes respiratory diseases and reproduction disorders in turkeys and chickens. The infection has considerable economic impact due to reduced meat and egg production. Because elimination programs are not feasible in a large number of poultry farms, vaccination remains the only effective measure of disease control.Differentiating vaccine strains from field isolates is necessary in the control of vaccination programs and diagnostics. The aim of this study was to develop a polymerase chain reaction (PCR) based mismatch amplification mutation assay (MAMA) for the discrimination of K vaccine strain (K 5831, Vaxxinova Japan K.K.).After determining the whole genome sequence (WGS) of the K strain, primers were designed to detect seven different vaccine-specific single nucleotide polymorphisms (SNPs). After evaluating preliminary results, the MAMA-K-fruA test detecting a single guanine-adenine substitution within the fruA gene (G88A) was found to be the most applicable assay to distinguish the K vaccine strain from field isolates.The detected K strain-specific SNP showed genetic stability...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research