Analysis of spatial relationships between CD8 and FoxP3 cells using digital imaging in head and neck squamous cell carcinoma

T cell-mediated anti-tumor immune responses are gated in part by the relative abundance of cytotoxic T cells (Teffs) and regulatory (Tregs) in the tumor microenvironment (TME) whereby Treg may impair the efficacy of Teffs. Tregs can affect Teff function by direct cell-to-cell contact or by secretion of soluble mediators, consistent with the hypothesis that proximity of Tregs to Teff within tumor tissues may have prognostic significance. Here we used a novel chromogenic multiplex assay to investigate the spatial relationship of these functionally diverse T cell subsets in HPV positive and HPV negative head and neck squamous cell carcinomas (HNSCCs).

Source: International Journal of Radiation Oncology * Biology * Physics - March 12, 2020 Category: Radiology Authors: M. Stewart, R. Stapp, D. Amin, R. Ganti, U. Nwagu, T. Richa, M. Crippen, R. Zinner, A. Luginbuhl, J. Johnson, V. Bar-Ad, U. Martinez-Outschoorn, C. Solomides, U. Rodeck, J.M. Curry Tags: Immunology and Immunotherapy Source Type: research