Synergistic amplification effect for electrochemiluminescence immunoassay based on dual coreactants coupling with resonance energy transfer.

Synergistic amplification effect for electrochemiluminescence immunoassay based on dual coreactants coupling with resonance energy transfer. Talanta. 2020 May 15;212:120798 Authors: Wang J, Wang H, Cao W, Zhang Q, Zhong W Abstract Sensitive electrochemiluminescence (ECL) bioanalysis could be achieved by exploring the amplification principle or strategy. This work described the first proof-of-concept study for coupling dual coreactants (K2S2O8 and H2O2) with localized surface plasmon resonance (LSPR) of silver nanoparticles (AgNPs) enhancement as an effective signal amplification strategy for ECL immunosensor. Specifically, AgNPs as an excellent conductor and nanocarriers were employed as the antibody (Ab2) immobilization film on the glassy carbon electrode (GCE) for the subsequent sandwich-type immunoreaction between the target protein and antibody (Ab1)-g-C3N4 resulted in the ECL generation. On the one hand, due to the perfect overlap between the plasmon absorption spectrum of AgNPs and the ECL emission spectrum of g-C3N4, a plasmon-enhanced ECL phenomenon was proved in g-C3N4-AgNPs system result from high efficient energy transfer. On the other hand, it was validated that the ECL signal of the constructed biosensor was greatly strengthened by K2S2O8 and H2O2 as dual coreactants, which was ca. 3.5 and 7 times stronger than that of K2S2O8 or H2O2 as an individual coreactant, respectively. Consequently, this synergistic amplified ECL ...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research