Radiation cystitis modeling: A comparative study of bladder fibrosis radio ‐sensitivity in C57BL/6, C3H, and BALB/c mice

Radiation cystitis, a severely debilitating condition characterized by fibrosis, vascular damage, and compromised bladder function, develops in cancer survivors who received pelvic radiation treatment. This is the first study to compare the effects of radiation on the bladder across different murine genetic backgrounds, which may explain differences in patients ’ sensitivity to developing radiation cystitis after radiation cancer treatment. AbstractA subset of patients receiving radiation therapy for pelvic cancer develop radiation cystitis, a complication characterized by mucosal cell death, inflammation, hematuria, and bladder fibrosis. Radiation cystitis can reduce bladder capacity, cause incontinence, and impair voiding function so severely that patients require surgical intervention. Factors influencing onset and severity of radiation cystitis are not fully known. We tested the hypothesis that genetic background is a contributing factor. We irradiated bladders of female C57BL/6, C3H, and BALB/c mice and evaluated urinary voiding function, bladder shape, histology, collagen composition, and distribution of collagen ‐producing cells. We found that the genetic background profoundly affects the severity of radiation‐induced bladder fibrosis and urinary voiding dysfunction. C57BL/6 mice are most susceptible and C3H mice are most resistant. Irradiated C57BL/6 mouse bladders are misshapen and express more abunda nt collagen I and III proteins than irradiated C3H and B...
Source: Physiological Reports - Category: Physiology Authors: Tags: ORIGINAL RESEARCH Source Type: research

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Radiation cystitis, a severely debilitating condition characterized by fibrosis, vascular damage, and compromised bladder function, develops in cancer survivors who received pelvic radiation treatment. This is the first study to compare the effects of radiation on the bladder across different murine genetic backgrounds, which may explain differences in patients ’ sensitivity to developing radiation cystitis after radiation cancer treatment. AbstractA subset of patients receiving radiation therapy for pelvic cancer develop radiation cystitis, a complication characterized by mucosal cell death, inflammation, hematuria,...
Source: Physiological Reports - Category: Physiology Authors: Tags: ORIGINAL RESEARCH Source Type: research
AbstractSeveral urinary disorders, including overactive bladder, urinary incontinence, and interstitial cystitis, are often characterized by negative urine cultures. The application of metagenomics (i.e., 16S rRNA microbial profiling or whole-genome shotgun sequencing) to urine samples has enabled the identification of previously undetected bacteria, contributing to the discovery and characterization of the urinary microbiome. The most frequent species isolated areLactobacillus (15%),Corynebacterium (14.2%),Streptococcus (11.9%),Actinomyces (6.9%), andStaphylococcus (6.9%). Although several studies are emerging in this con...
Source: Targeted Oncology - Category: Cancer & Oncology Source Type: research
Kate V. Meriwether1*, Zhenmin Lei1, Rajbir Singh2, Jeremy Gaskins3, Deslyn T. G. Hobson1 and Venkatakrishna Jala2 1Department of Obstetrics and Gynecology, University of Louisville, Louisville, KY, United States 2Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, United States 3Department of Bioinformatics and Biostatistics, University of Louisville, Louisville, KY, United States Interstitial cystitis/bladder pain syndrome (ICBPS) may be related to an altered genitourinary microbiome. Our aim was to compare the vaginal and urinary microbiomes b...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
This study aims to investigate contouring and dose evaluation of sub-structures potentially responsible for urinary morbidity after radiochemotherapy and Image Guided Adaptive Brachytherapy (IGABT) in locally advanced cervical cancer (LACC).
Source: Physica Medica: European Journal of Medical Physics - Category: General Medicine Authors: Source Type: research
ConclusionsIn this review, we will present current and advanced therapeutic strategies for RC to help cancer survivors deal with long-term bladder health issues.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
This study aims to investigate contouring and dose evaluation of several bladder sub-structures potentially responsible for urinary morbidity after radiochemotherapy and Image Guided Adaptive Brachytherapy (IGABT) in locally advanced cervical cancer (LACC).
Source: Physica Medica: European Journal of Medical Physics - Category: General Medicine Authors: Source Type: research
CONCLUSIONS: In case of prostate carcinomas, serious complications can be avoided by the improvement of diagnostic and therapeutic options. Synthesis of data could be more successful if they were analyzed in the light of previous experiences. Orv Hetil. 2018; 159(32): 1317-1325. PMID: 30078360 [PubMed - in process]
Source: Orvosi Hetilap - Category: General Medicine Authors: Tags: Orv Hetil Source Type: research
We presented an interesting case with a 36-year-old man who's symptoms mimic acute prostatitis but there was no positive pathogen been cultured. The computed tomography (CT) findings revealed asymmetrical bladder wall thickening, which misleading us to the impression of bladder cancer. After the cystoscopy with bladder biopsy, the pathology revealed severe inflammation without malignancy. After that, we prescribed anticholinergic agent, beta-3 agonist and nonsteroidal anti-inflammatory drug (NSAID) for him, but in vain. Diagnoses: Erosive cystitis with prominent infiltration by eosinophils, lymphocytes, neutrophils and ...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
Radiation cystitis (RC), characterized by suprapubic pain, hematuria, nocturia, incontinence and frequency, is a debilitating bladder condition caused by radiation therapy directed to the pelvic area. Approximately 5-10% of cancer survivors with a history of pelvic radiation treatment will develop RC. However, the lack of knowledge on the histological and molecular drivers of RC has limited the development of effective therapies. A major histological hallmark of RC is a damaged vascular network that results in varying degrees of hemorrhaging within the bladder.
Source: The Journal of Urology - Category: Urology & Nephrology Authors: Tags: Infections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I Source Type: research
(1) Objectives: To investigate whether urinary levels of macrophage migration inhibitory factor (MIF) are elevated in Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS) patients with Hunner lesions and also whether urine MIF is elevated in other forms of inflammatory cystitis. (2) Methods: Urine samples were assayed for MIF by ELISA. Urine samples from three female groups were examined: IC/BPS patients without (N=55) and with Hunner lesions (N=43); Non-IC/BPS patients (N=100; control group; no history of IC/BPS; cancer or recent bacterial cystitis).
Source: Urology - Category: Urology & Nephrology Authors: Tags: Female Urology, Urodynamics, Incontinence, and Pelvic Floor Reconstructive Surgery Source Type: research
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