MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles.

MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles.
Methods Mol Biol. 2020;2126:107-116
Authors: Zhu W, Xu Y, Jin R, Wu C, Ai H
Abstract
Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79-90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.
PMID: 32112383 [PubMed - as supplied by publisher]

Source: Mol Biol Cell - March 2, 2020 Category: Molecular Biology Authors: Zhu W, Xu Y, Jin R, Wu C, Ai H Tags: Methods Mol Biol Source Type: research