Aligned nanofibers of decellularized muscle extracellular matrix for volumetric muscle loss

In this study, we investigate the extent to which this electrospun scaffold can supp ort functional muscle regeneration in a murine model of VML. Experimental groups included no treatment, pure PCL treated, and PCL:D‐ECM (50:50 blend) treated VML defects. The PCL:D‐ECM scaffold treated VML muscles supported increased activity of anti‐inflammatory M2 macrophages (arginase+) at Day 28, compared to other experimental groups. Increased myofiber (MHC+) regeneration was observed histologically at both Days 7 and 28 post ‐trauma in blend scaffold treated group compared to PCL treated and untreated groups. However, improvements in muscle weights and force production were not observed. Future studies would evaluate muscle function at longer time‐points post‐VML injury to allow sufficient time for reinnervation o f regenerated muscle fibers.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research