Class III β-tubulin expression during hard tissue formation in developing mouse teeth

In this study, we investigated TUBB3 expression in the developing mouse teeth using immunohistochemistry. Similar to previous research, immunohistochemistry with TUBB3-specific antibodies showed immunoreactivity in the nerve fiber around the tooth germ. However, TUBB3 immunoreactivity was minimal or not seen at all in the tooth germ at early cytological and histological differentiation stages of developing teeth. However, the immunoreactivity was intense in odontoblasts during dentin formation and in most ameloblasts at the secretory stage. Intense immunoreactivity was observed only in some ameloblasts at the maturation stage. Similar to the immunoreactivity in ameloblasts and odontoblasts, the immunoreactivity was intense in cementoblasts during cementum formation. The spaciotemporal profiles of TUBB3 expression suggested that TUBB3 may be correlated to some cellular process at the mineralization stage of tooth development.
Source: Pediatric Dental Journal - Category: Dentistry Source Type: research