Molecular epidemiology of Enterocytozoon bieneusi and Encephalitozoon sp., among immunocompromised and immunocompetent subjects in Iran

Publication date: Available online 21 January 2020Source: Microbial PathogenesisAuthor(s): Kambiz Karimi, Hamed Mirjalali, Maryam Niyyati, Ali Haghighi, Mohammad Amin Pourhosseingholi, Meysam Sharifdini, Nosratollah Naderi, Mohammad Reza ZaliAbstractIntestinal microsporidiosis is known as an opportunistic infection in immunocompromised patients. The current study aimed to investigate intestinal microsporidia infection in human subjects with/without immunodeficiency. Totally, 600 stool samples were collected from immunocompromised (254) and immunocompetent (346) subjects. DNA extraction was performed and the SSU rRNA and ITS genes were amplified to detect and characterize microsporidia and the relevant genotypes. Phylogenetic trees were drawn using MEGA7 software to illustrate correlation between isolates. From 600 enrolled subjects, 283 and 317 were male and female, respectively. The average age ±SD of all tested subjects was 28.85 ± 26.92. The results of PCR revealed E. bieneusi and Encephalitozoon sp., among 10/600 (1.67%) and 26/600 (4.33%) of samples, respectively. Accordingly, E. bieneusi was seen among 4/346 (1.15%), 1/53 (1.88%), 3/124 (2.42%) and 2/63 (3.17%), and Encephalitozoon sp., were detected from 17/346 (4.91%), 3/53 (5.36%), 4/124 (3.22%) and 2/63 (3.17%) of healthy subjects, RA patients, cancer patients and transplantation recipients, respectively. Statistical significant correlation was not seen between the presence of microsporidia and age, gender, st...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research