GSE110680 Inhibiting WNT and NOTCH in renal cancer stem cells: implications for human patients

Contributors : Annika Fendler ; Kun Son ; Wei Chen ; Walter BirchmeierSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe detected and characterized CXCR4+MET+CD44+ cancer stem cells (CSCs) that were quantitatively correlated with aggressive tumors. Transcriptional profiling and single-cell sequencing revealed a strong activation of WNT and NOTCH signaling in these CSCs. CEL-Seq was performed to study heterogeneity of the CXCR4+MET+CD44+ population.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

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(Max Delbr ü ck Center for Molecular Medicine in the Helmholtz Association) Scientists at the MDC have discovered stem cells responsible for the most common form of kidney cancer. The team of Walter Birchmeier has found a way to block the growth of these tumors in three models of the disease.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Contributors : Annika Fendler ; Jonas Busch ; Klaus Jung ; Annika Wulf-Goldenberg ; Bettina Erg ün ; Simone Jung ; Wei Chen ; Walter BirchmeierSeries Type : Expression profiling by arrayOrganism : Homo sapiensCurrent therapies for metastasized kidney cancer in humans include treatments with multi-tyrosine kinase inhibitors, monoclonal antibodies against VEGF or inhibitors of mTOR. While many tumors initially respond to these treatments, nearly all resume their progression within two years. Here we characterize CXCR4+MET+CD44+ cancer stem cells in clear cell renal cancer (ccRCC), whose presence represents a major subgr...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
This study aims to compare the biology and telomerase activity of CSCs to parental cells (PCs) in renal cancer. Renal CSCs were enriched from the ACHN cell line using a sphere culture system. Spheroid-derived cells (SDCs) and their adherent counterparts were compared with respect to their colony and sphere formation, expression of putative CSC markers, tumorigenicity in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, and invasiveness. The expression of genes associated with CSCs, stemness, EMT, apoptosis, and ABC transporters was also compared between the two populations using quantitative real-time PC...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
We reported on a 54-year-old patient with a diagnosis of grade 2 follicular lymphoma (FL) and concomitant renal cancer. After the nephrectomy, the patient underwent two lines of chemoimmunotherapy, autologous stem cell transplant, obtained a complete response with idelalisib and underwent an allogeneic transplant (alloSCT).
Source: Clinical Lymphoma, Myeloma and Leukemia - Category: Hematology Authors: Tags: Case Report Source Type: research
Metastatic renal cell carcinoma cells growing in 3D on poly‑D‑lysine or laminin present a stem‑like phenotype and drug resistance. Oncol Rep. 2019 Sep 18;: Authors: Brodaczewska KK, Bielecka ZF, Maliszewska-Olejniczak K, Szczylik C, Porta C, Bartnik E, Czarnecka AM Abstract 3D spheroids are built by heterogeneous cell types in different proliferative and metabolic states and are enriched in cancer stem cells. The main aim of the study was to investigate the usefulness of a novel metastatic renal cell carcinoma (RCC) 3D spheroid culture for in vitro cancer stem cell physiology research and dr...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
In this study, we determine whether transient reintroduction of embryonic stem cell cycle miR-294 promotes cardiomyocyte cell cycle reentry enhancing cardiac repair after myocardial injury. A doxycycline-inducible AAV9-miR-294 vector was delivered to mice for activating miR-294 in myocytes for 14 days continuously after myocardial infarction. miR-294-treated mice significantly improved left ventricular functions together with decreased infarct size and apoptosis 8 weeks after MI. Myocyte cell cycle reentry increased in miR-294 hearts parallel to increased small myocyte number in the heart. Isolated adult myocytes from miR-...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Trichloroethylene (TCE) and inorganic arsenic (iAs) are environmental contaminants that can target the kidney. Chronic exposure to TCE is associated with increased incidence of renal cell carcinoma, while co-exposure to TCE and iAs likely occurs in exposed human populations, such as those near Superfund sites. In order to better understand the kidney health consequences of TCE and/or iAs exposure, a genetically heterogeneous mouse population derived from FVB/NJ and CAST/EiJ mouse strains and deficient for multidrug resistance genes (Abcb1atm1Bor, Abcb1btm1Bor) was chronically exposed for 52-weeks to varying concentrations ...
Source: G3: Genes Genomes Genetics - Category: Genetics & Stem Cells Authors: Tags: Investigations Source Type: research
Discussion MDSCs violently emerge in pathological conditions in an attempt to limit potentially harmful immune and inflammatory responses. Mechanisms supporting their expansion and survival are deeply investigated in cancer, in the perspective to reactivate specific antitumor responses and prevent their contribution to disease evolution. These findings will likely contribute to improve the targeting of MDSCs in anticancer immunotherapies, either alone or in combination with immune checkpoint inhibitors. New evidence indicates that the expansion of myeloid cell differentiation in pathology is subject to fine-tuning, as its...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Hui Zhou, Xiaoyan Fu, Qian Li and Ting Niu* Department of Hematology and Research Laboratory of Hematology, West China Hospital, Sichuan University, Chengdu, China Background: Immune checkpoint inhibition therapy with monoclonal antibody against programmed cell death protein 1 (PD-1), including nivolumab and pembrolizumab, has demonstrated powerful clinical efficacy in the treatment of advanced cancers. However, there is no evidence-based systematic review on the safety and efficacy of anti-PD-1 antibody in treating lymphoma. Methods: To evaluate the safety and efficacy of nivolumab/pembrolizumab, we analyzed clin...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Yong Zhao1, Peijuan Liu1, Zhiqian Xin1, Changhong Shi1, Yinlan Bai2, Xiuxuan Sun3, Ya Zhao1, Xiaoya Wang1,4, Li Liu1,5, Xuan Zhao1,4, Zhinan Chen3* and Hai Zhang1,6* 1Laboratory Animal Center, Air Force Medical University, Xi’an, China 2Department of Microbiology, Air Force Medical University, Xi’an, China 3Department of Cell Biology, National Translational Science Center for Molecular Medicine, Air Force Medical University, Xi’an, China 4College of Veterinary Medicine, Northwest A&F University, Yangling, China 5Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Nor...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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