In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4

Publication date: Available online 17 January 2020Source: Veterinary ParasitologyAuthor(s): Pengfei Zhao, Yuncan Li, Yanqin Zhou, Junlong Zhao, Rui FangAbstractEimeria tenella, belonging to protozoon, is the causative agent of cecal coccidiosis in chicken and causes enormous impacts for poultry industry. The surface antigens of apicomplexan parasites function as attachment and invasion in host-parasite interaction. Meanwhile, host immune response is triggered as a result of parasitic invasion. Immunogenicity and potency as a vaccinal candidate antigen of E. tenella surface antigen 4 (EtSAG4) have been unknown. Therefore, a gene segment of E. tenella EtSAG4 was amplified and transplanted to pET28a prokaryotic vector for recombinant protein expression. Similarly, pEGFP-N1 eukaryotic vectors with EtSAG4 gene segment (pEGFP-N1-EtSAG4) amplified in 293 T cells as DNA vaccines. Reverse transcription-polymerase chain reaction (RT-PCR) assay and western blot analysis were used to demonstrate successful expressions of EtSAG4 in Escherichia coli or 293 T cells. Subsequently, animal experiments (72 cobb broilers) were performed to evaluate immunoprotective between recombinant protein and DNA vaccine of E. tenella EtSAG4 using different immunizing doses (50 or 100 μg), respectively. Serum from chickens infected with E. tenella identified recombinant EtSAG4 (rEtSAG4) protein. Chickens vaccinated with either rEtSAG4 protein or pEGFP-N1-EtSAG4 plasmids both shown a s...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research

Related Links:

Publication date: Available online 14 February 2020Source: Fish &Shellfish ImmunologyAuthor(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total molecular mass of 11,182 Da was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrophotometry. The N-t...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
CONCLUSIONS: The in vitro and in vivo giardicidal activity of CMC-20 suggests its potential use in the treatment of giardiasis. PMID: 32049098 [PubMed - in process]
Source: Memorias do Instituto Oswaldo Cruz - Category: Infectious Diseases Authors: Tags: Mem Inst Oswaldo Cruz Source Type: research
AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
Publication date: Available online 11 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Ankit Gupta, Kirti Gupta, Saman HabibAbstractThe YihA TRAFAC GTPases are critical for late-stage assembly of the ribosomal large subunit (LSU). In order to explore biogenesis of the reduced organellar ribosomes of the malaria parasite, we identified three nuclear-encoded homologs of YihA in Plasmodium falciparum. PfYihA1 targeted to the parasite apicoplast, PfYihA2 to the mitochondrion, and PfYihA3 was found in both the apicoplast and cytosol. The three PfYihA, expressed as recombinant proteins, were active GTPases a...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research
Conclusions/SignificanceAs37 is anA.suum expressed immunodominant antigen that elicited significant protective immunity in mice when formulated with AddaVax™. As37 is highly conserved in other STHs, but not in humans, suggesting it could be further developed as a pan-helminth vaccine against STH co-infections.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Medina-Franco Chale-Dzul Moo-Puc Navarrete-Vázquez We prepared a series of 10 carbamates derivatives based on two common antiprotozoal drugs: metronidazole (1–5) and secnidazole (6–10). The compounds were tested in vitro against a set of two amitochondriate protozoa: Giardia duodenalis and Trichomonas vaginalis. Compounds 1–10 showed strong antiprotozoal activities, with potency values in the low micromolar-to-nanomolar range, being more active than their parent drugs. Metronidazole carbamate (1) was the most active of the series, with nanomolar activities against G. duod...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: Available online 8 February 2020Source: Veterinary MicrobiologyAuthor(s): Fang Liu, Yue Xie, Anne M. Zajac, Yan Hu, Raffi V. Aroian, Joseph F. Urban, Robert W. LiAbstractParasitic infections are associated with profound changes in the structure and function of the gut microbiome in various host-parasite systems. Here we examined the microbial composition and function in the abomasum, proximal colon and feces of Haemonchus contortus-infected goats after a partial anthelmintic drug clearance. A single-dose treatment of H. contortus-infected goats with Cydectin (moxidectin) resulted in an 83.9% and 61.8% red...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 5 February 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Emily Jenkins, Amber-Lynn Backwell, Jennifer Bellaw, Julie Colpitts, Alice Liboiron, David McRuer, Sarah Medill, Sarah Parker, Todd Shury, Martha Smith, Christina Tschritter, Brent Wagner, Jocelyn Poissant, Philip McLoughlinAbstract:Sable Island, Nova Scotia, Canada hosts one of few natural populations of feral horses (Equus caballus) never exposed to anthelmintics. Coproculture revealed cyathostomes, Strongylus equinus, S. edentatus, and S. vulgaris, with S. equinus (unusually) dominating in a...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
AbstractBlastocystis is a parasite with a worldwide distribution and a varying prevalence in different countries. The pleomorphic nature of the protozoon and the lack of understanding a possible pathogenesis have led to confusion regarding its clinical significance. The aim of the study was to shed light on clinical characteristics of pediatric patients in Swiss children with a positive stool sample forBlastocystis, in order to provide recommendations for a practical approach for the clinician to know whom, when, and how to test. This is a retrospective study of pediatric patients, whose stool has been tested positive forB...
Source: European Journal of Pediatrics - Category: Pediatrics Source Type: research
More News: Gastroenteritis | Genetics | Parasitic Diseases | Parasitology | Transplants | Vaccines | Veterinary Research | Veterinary Vaccinations