Modelling the development of anthelmintic resistance in cyathostomin parasites: the importance of genetic and fitness parameters
Publication date: Available online 17 April 2019Source: Veterinary ParasitologyAuthor(s): Christian W. Sauermann, Martin K. Nielsen, Dongwen Lou, Dave M. LeathwickAbstractPreviously described models for the free-living and parasitic phases of the cyathostomin life-cycle were combined into a single model for the complete life-cycle. The model simulates a single free-living population on pasture utilising parasite egg output from the horses and localised temperature and rainfall data to estimate infective larval density on herbage. Multiple horses of different ages are possible, each with an individualised anthelmintic treat...
Source: Veterinary Parasitology - April 18, 2019 Category: Veterinary Research Source Type: research

Similar levels of diversity in the gene encoding the p67 sporozoite surface protein of Theileria parva are observed in blood samples from buffalo and cattle naturally infected from buffalo
Publication date: Available online 13 April 2019Source: Veterinary ParasitologyAuthor(s): Tatjana Sitt, Sonal Henson, W. Ivan Morrison, Philip ToyeAbstractTheileria parva is a tick-transmitted, apicomplexan protozoan found in buffalo (Syncerus caffer) and cattle in eastern, central and southern Africa. The parasite causes a fatal, lymphoproliferative disease in susceptible cattle. Previous studies have shown that the parasites in buffalo comprise a more heterogeneous population than those in cattle, which has led to the concept that the population of parasites circulating in cattle represents a restricted subpopulation of ...
Source: Veterinary Parasitology - April 15, 2019 Category: Veterinary Research Source Type: research

Salivary IgA: a biomarker for resistance to Teladorsagia circumcincta and a new Estimated Breeding Value
In this study salivary IgA responses were measured in over 5000 animals against L3 antigen by Enzyme Linked Immunosorbent Assay (ELISA). Antigen-specific IgA levels were negatively correlated with FEC (r=-0.26, SE = 0.02) and were heritable (h2 = 0.16, SE = 0.04) indicating that they can be used to identify resistant animals suitable for inclusion in selective breeding programs. Antigen-specific IgA responses were not negatively correlated with muscle depth. Our analyses indicate that selection for T. circumcincta L3 antigen-specific IgA is possible without impacting on the production traits for...
Source: Veterinary Parasitology - April 15, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: April 2019Source: Veterinary Parasitology, Volume 268Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - April 11, 2019 Category: Veterinary Research Source Type: research

Toxoplasma gondii tissue cyst formation and density of tissue cysts in shoulders of pigs 7 and 14 days after feeding infected mice tissues
Publication date: Available online 10 April 2019Source: Veterinary ParasitologyAuthor(s): S. Rani, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, M. Sadler, O.C.H. Kwok, A.K. Pradhan, D.E. Hill, J.F. Urban, J.P. DubeyAbstractAmong the meat sources of Toxoplasma gondii, pork is considered important in the epidemiology of toxoplasmosis in the USA. How soon after infection T. gondii forms tissue cysts in pork is unknown. In the present study, eight serologically negative ˜3 months old pigs were fed mouse tissues infected with VEG (Type III) strain of T. gondii and euthanized 7 (4 pigs) and 14 days (4 pigs) post...
Source: Veterinary Parasitology - April 10, 2019 Category: Veterinary Research Source Type: research

Efficacy and safety of a new topical formulation containing selamectin and sarolaner in the prevention of heartworm disease and the treatment of roundworm infection in cats presented as veterinary patients in Japan
Publication date: Available online 8 April 2019Source: Veterinary ParasitologyAuthor(s): Wakako Yonetake, Takeshi Fujii, Masaya Naito, Steven Maeder, Douglas RuggAbstractThe efficacy and safety of a new topical formulation containing selamectin plus sarolaner (Revolution® Plus / Stronghold® Plus, Zoetis) was evaluated for the prevention of heartworm (Dirofilaria immitis) disease and the treatment of roundworm infection in cats enrolled as veterinary patients in two field studies conducted in Japan.In the heartworm field study, 91 cats negative for D. immitis antigen and anti-D. immitis antibody were enrolled and re...
Source: Veterinary Parasitology - April 10, 2019 Category: Veterinary Research Source Type: research

The effect of climate, season, and treatment intensity on anthelmintic resistance in cyathostomins: a modelling exercise
This study made use of a computer model of the entire cyathostomin life-cycle to evaluate the influence of climate and seasonality on the development of anthelmintic resistance in cyathostomin parasites. Furthermore, the study evaluated the impact of recommended programs involving selective anthelmintic therapy on delaying anthelmintic resistance development. All simulations evaluated the use of a single anthelmintic (i.e., ivermectin) over the course of 40 model years. The study made use of weather station data representing four different climatic zones: a cold humid continental climate, a temperate oceanic climate, a col...
Source: Veterinary Parasitology - April 8, 2019 Category: Veterinary Research Source Type: research

Corrigendum to “Trichinella spiralis prevalence among wildlife of a boreal region rapidly reduced in the absence of spillover from the domestic cycle” [Vet. Parasitol. 262C (2018) 1–5]
Publication date: May 2019Source: Veterinary Parasitology, Volume 269Author(s): Antti Oksanen, Maria Interisano, Marja Isomursu, Petra Heikkinen, Daniele Tonanzi, Leena Oivanen, Edoardo Pozio (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - April 8, 2019 Category: Veterinary Research Source Type: research

A probe-based real-time PCR assay for the detection of Neospora caninum in clinical samples from cattle
In conclusion, the probe-based real-time PCR assay facilitates accurate and rapid detection of N. caninum from abortions in cattle. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - April 5, 2019 Category: Veterinary Research Source Type: research

Relationship between pharmacokinetics of ivermectin (3.15%) and its efficacy to control the infestation with the tick Rhipicephalus (Boophilus) microplus in cattle
This study aimed to determine the relationship between the variation in plasma concentration of ivermectin 3.15% over time and its efficacy against the cattle tick Rhipicephalus (Boophilus) microplus. In addition, a trial was conducted to infer if the application of successive treatments with ivermectin 3.15% could affect its accumulation in cattle. A noticeable variation of ivermectin plasma concentration was observed among the treated heifers. However, these differences did not have a significant effect onthe therapeutic efficacy of the treatment at the end of the trial. No significant differenceswere observed in the lev...
Source: Veterinary Parasitology - April 2, 2019 Category: Veterinary Research Source Type: research

Tissue (re)distribution of Trypanosoma equiperdum in venereal infected and blood transfused horses
This study describes the distribution of the parasite after infection by artificial insemination (AI) or blood transfusion. Mares (N = 4) were artificially inseminated with T. equiperdum spiked semen whereas stallions (N = 4) were infected by blood transfusion. The course of the disease was monitored by parasitological (Woo) and molecular (PCR) tests and clinical signs and haematological parameters were recorded. At 120 days post infection, horses had a full necropsy, histopathology and PCR. A similar pattern of parasitaemia, disease progression and tissue distribution were seen in all horses. E...
Source: Veterinary Parasitology - March 29, 2019 Category: Veterinary Research Source Type: research

Th2-related cytokines are associated with Fasciola gigantica infection and evasion in the natural host, swamp buffalo
In this study, native swamp buffaloes were each infected with 500 viable F. gigantica metacercariae. Mesenteric lymph node (MLN), hepatic lymph node (HLN), spleen, and serum samples were collected from control and infected buffaloes at 3, 10, 28, 42, 70, and 98 days post-infection (DPI). The mRNA expression levels of the Th1- and Th2-related cytokines IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p40, IFN-γ, TNF-α, and CD4 were measured during different infection stages in the MLNs, spleens, and HLNs using quantitative real-time PCR (qRT-PCR). Levels of the specific anti-ESP isotype antibodies IgG, IgG1, and IgG2 were us...
Source: Veterinary Parasitology - March 27, 2019 Category: Veterinary Research Source Type: research

Effect of strategic deworming on Ascaris suum exposure and technical performance parameters in fattening pigs
Publication date: Available online 23 March 2019Source: Veterinary ParasitologyAuthor(s): Elise Vandekerckhove, Johnny Vlaminck, Rubén del Pozo Sacristán, Peter GeldhofAbstractThe aim of this study was to investigate the effect of a strategic deworming program on Ascaris suum infection levels and technical performance parameters in fattening pigs. Eighteen fattening stables were selected and divided into two groups. Group 1 consisted of 9 stables in which the fattening pigs tested seropositive for Ascaris, indicative for the presence of Ascaris eggs in the stable, whereas group 2 consisted of 9 stables in whi...
Source: Veterinary Parasitology - March 24, 2019 Category: Veterinary Research Source Type: research

Chemical characterization and acaricidal activity of Drimia maritima (L) bulbs and Dittrichia viscosa leaves against Dermanyssus gallinae
Publication date: Available online 19 March 2019Source: Veterinary ParasitologyAuthor(s): Wafa Rhimi, Issam Ben salem, Antonio Camarda, Mouldi Saidi, Abdennacer Boulila, Domenico Otranto, Claudia CafarchiaAbstractThe emergence of resistance to chemical acaricides in Dermanyssus gallinae, together with their toxicity and high costs, has prompted investigations into the use of plant extracts as alternatives to chemical acaricidal treatments. Drimia maritima bulbs and Dittrichia viscosa (D. viscosa) leaf extracts were here characterized by HPLC-PDA-ESI-MS/MS, and their toxicity against D. gallinae was evaluated using contact ...
Source: Veterinary Parasitology - March 20, 2019 Category: Veterinary Research Source Type: research

A model for the dynamics of the parasitic stages of equine cyathostomins
Publication date: Available online 18 March 2019Source: Veterinary ParasitologyAuthor(s): Dave M. Leathwick, Christian W. Sauermann, Craig R. Reinemeyer, Martin K. NielsenAbstractA model was developed to reproduce the dynamics of the parasitic stages of equine cyathostomins. Based on a detailed review of published literature, a deterministic simulation model was constructed using the escalator box-train approach, which allows for fully-overlapping cohorts of worms and approximately normally distributed variations in age/size classes. Key biological features include a declining establishment of ingested infective stage larv...
Source: Veterinary Parasitology - March 19, 2019 Category: Veterinary Research Source Type: research

An oral dose of Fluralaner administered to dogs kills pyrethroid-resistant and susceptible Chagas disease vectors for at least four months
Publication date: Available online 18 March 2019Source: Veterinary ParasitologyAuthor(s): M.A. Laiño, M.V. Cardinal, G.F. Enriquez, A. Alvedro, M.S. Gaspe, R.E. GürtlerABSTRACTNew vector control tools that can fit into a broader integrated vector management strategy are notably lacking. We conducted a seven-month randomized trial to assess the efficacy of a single oral dose of Fluralaner (Bravecto®) administered to dogs on the blood-feeding success, engorgement levels and mortality of pyrethroid-resistant and -susceptible Triatoma infestans third- and fifth-instar nymphs. The trial included 10 Fluralaner-tr...
Source: Veterinary Parasitology - March 19, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: March 2019Source: Veterinary Parasitology, Volume 267Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - March 14, 2019 Category: Veterinary Research Source Type: research

Acaricidal efficacy of deltamethrin-zinc oxide nanocomposite on Rhipicephalus (Boophilus) annulatus tick
In conclusion, deltamethrin-ZnO nanocomposite was the most effective adulticide and larvicide against R. (B.) annulatus. To the best of our knowledge, this is the first report for using deltamethrin-ZnO NPs as an acaricide. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - March 12, 2019 Category: Veterinary Research Source Type: research

Efficacy of a new topical formulation of selamectin plus sarolaner for the control of fleas and ticks infesting cats in Japan
Publication date: Available online 8 March 2019Source: Veterinary ParasitologyAuthor(s): Wakako Yonetake, Takeshi Fujii, Masaya Naito, Andrew Hodge, Steven Maeder, Douglas RuggAbstractThe efficacy of a single application of a new topical formulation containing selamectin plus sarolaner (Revolution® Plus / Stronghold® Plus, Zoetis) was evaluated against fleas and ticks infesting cats enrolled as veterinary patients in two field studies conducted in Japan and against Haemaphysalis longicornis ticks on cats in a laboratory study.In the laboratory study, sixteen cats were ranked based on pre-treatment tick counts and a...
Source: Veterinary Parasitology - March 10, 2019 Category: Veterinary Research Source Type: research

Propagation of Giardia duodenalis cysts in Immunosuppressed CF-1 Mice
This study developed and evaluated Giardia duodenalis cyst propagation using a dexamethasone immuno-suppressed CF-1 mouse model as an alternative to a previously described Mongolian gerbil model. The CF-1 mouse model shed significantly more cysts per animal during a 16 to18 h collection period compared to the gerbil (averages: 7.8 × 106 cysts/CF-1 mouse and 2.5 × 106 cysts/gerbil). In addition, the patency period for this model differed from both G. muris in mice and G. duodenalis in gerbils in that cysts were shed continuously for over 20 days. Results further showed that the...
Source: Veterinary Parasitology - March 6, 2019 Category: Veterinary Research Source Type: research

Anthelmintic efficacy of single active and combination products against commonly occurring parasites in foals
Publication date: Available online 1 March 2019Source: Veterinary ParasitologyAuthor(s): L.H. Morris, S. Colgan, D.M. Leathwick, M.K. NielsenAbstractParasite control in foals is complicated by the concurrent presence of biologically diverse parasites with differing levels of anthelmintic resistance. Several combination anthelmintic products are available for use in horses, but information on their efficacies against important equine parasites is scarce. Two trials were performed in New Zealand during 2008 and 2011 on four different farms with substantially different anthelmintic treatment histories. The first trial evaluat...
Source: Veterinary Parasitology - March 5, 2019 Category: Veterinary Research Source Type: research

Fasciola spp. in Armenia: Genetic diversity in a global context
In this study, livestock in central Armenia was surveyed for fasciolosis, the causative species were determined and the genetic diversity of both species was estimated. Total prevalence in sheep (1,794), cattle (324) and goats (9) was 21.2 %, 15.7 % and 44.4 %, respectively. After morphological identification and sequencing of a mitochondrial (nad1) and a nuclear marker gene (28S rRNA), 62 collected specimens were allocated to F. hepatica (n = 55) and F. gigantica (n = 7). Intraspecific diversity was evaluated for the complete nad1 gene, resulting in 29 haplotypes of F. hepatica and six haplotyp...
Source: Veterinary Parasitology - March 5, 2019 Category: Veterinary Research Source Type: research

A high throughput deep amplicon sequencing method to show the emergence and spread of Calicophoron daubneyi rumen fluke infection in United Kingdom cattle herds
Publication date: Available online 1 March 2019Source: Veterinary ParasitologyAuthor(s): Neil D. Sargison, Kashif Shahzad, Stella Mazeri, Umer ChaudhryAbstractThe prevalence of C. daubneyi infection in the United Kingdom has increased, but despite the potential for rumen flukes to cause production loss in ruminant livestock, understanding of their emergence and spread is poor. Here we describe the development of a method to explore the multiplicity of C. daubneyi infection and patterns of the parasite’s emergence and spread, based on Illumina MiSeq deep sequencing of meta barcoded amplicons of a fragment of the cytoc...
Source: Veterinary Parasitology - March 5, 2019 Category: Veterinary Research Source Type: research

Inflammasome activation restrains the intracellular Neospora caninum proliferation in bovine macrophages
In conclusion, this study is the first report on the role of the bovine inflammasome in restraining intracellular N. caninum replication and suggests that the bovine inflammasome may be a potential target for future development of drugs or vaccines against N. caninum infection in cattle. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - March 5, 2019 Category: Veterinary Research Source Type: research

Comment on the paper “Chemical analyses and anthelmintic effects of Artemisia campestris essential oil”
Publication date: March 2019Source: Veterinary Parasitology, Volume 267Author(s): Emelda Ongo (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 20, 2019 Category: Veterinary Research Source Type: research

Neospora GRA6 possesses immune-stimulating activity and confers efficient protection against Neospora caninum infection in mice
In this study, the immune-stimulatory effect of recombinant Neospora caninum dense granule protein 6 (NcGRA6) was confirmed via its triggering of IL-12p40 production in murine macrophages. BALB/c mice were immunized with recombinant NcGRA6 fused with glutathione S-transferase (GST) protein with or without oligomannose-coated-liposomes (OMLs) as the potential adjuvant. Specific IgG1 antibody production was observed from 21 and 35 days after the first immunization in NcGRA6+GST- and NcGRA6+GST-OML-immunized mice, respectively. However, specific IgG2a was detected 1 week after the infection, and IgG2a levels of the NcGRA6+GST...
Source: Veterinary Parasitology - February 17, 2019 Category: Veterinary Research Source Type: research

Flow cytometry evaluation of in vitro susceptibility of bovine isolates of Tritrichomonas foetus to metronidazole
Publication date: Available online 16 February 2019Source: Veterinary ParasitologyAuthor(s): María Belén Rivero, Melchor Emilio Luque, Maria Eugenia Abdala, Bruno Elías Luna, David Di Lullo, Pedro Gabriel Carranza, Fernando David RiveroAbstractBovine Trichomonosis, an endemic sexually transmitted disease in countries with extensive livestock and natural service, represents one of the most common causes of reproductive failure. 5-nitroimidazoles and their derivatives are used for its treatment, mainly metronidazole (Mz). The emergence of resistance mechanisms adopted by the parasites against the drug an...
Source: Veterinary Parasitology - February 17, 2019 Category: Veterinary Research Source Type: research

Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples
This study demonstrated that all the PNA detection methods tested with H. contortus eggs generated fluorescence intensities (FIs) that were significantly above the autofluorescence generated by the eggs among the three different fluorescence filters. Fluorescence intensities from PNA directly conjugated to either the FITC or rhodamine fluorophores were not different, but the lower autofluoresence in the rhodamine-type filter will enable this fluorophore to be detected more efficiently. Use of biotinylated PNA combined with streptavidin-conjugated to synthetic fluorophores (Alexa Fluor 405, 488 and 546) significantly increa...
Source: Veterinary Parasitology - February 17, 2019 Category: Veterinary Research Source Type: research

Protective efficacy of liver fluke DNA vaccines: A systematic review and meta-analysis: Guiding novel vaccine development
Publication date: Available online 16 February 2019Source: Veterinary ParasitologyAuthor(s): Rama Jayaraj, Chellan Kumarasamy, Luke Norbury, David Piedrafita, Peter SmookerAbstractThe immunogenicity and efficacy of Fasciola DNA vaccines have not yet been comprehensively summarised in the form of a systematic review and meta-analysis. Though multiple vaccine studies with respect to Fasciola vaccines exist, the variance in the experimental parameters has made comparison difficult.We conducted a bibliographic database search in Scopus, PubMed, Science Direct, Cochrane Library, EMBASE and Web of Science databases, limited to p...
Source: Veterinary Parasitology - February 17, 2019 Category: Veterinary Research Source Type: research

Dirofilaria immitis and D. repens in dog and cat: a questionnaire study in Italy
This study show that veterinary facilities ians working in an endemic area for D. immitis and D. repens are aware of recent developments in adulticide therapy. Results would suggest however that diagnosis is not always carried out according to what is currently recommended by international guidelines and that the timing for starting prevention may not be optimal. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 14, 2019 Category: Veterinary Research Source Type: research

Acaricidal activity and enzyme inhibitory activity of active compounds of essential oils against Psoroptes cuniculi
Publication date: Available online 12 February 2019Source: Veterinary ParasitologyAuthor(s): Xiao-Fei Shang, Li-Xia Dai, Ying-Qian Liu, Zhong-Min Zhao, Jun-Cai Li, Guan-Zhou Yang, Cheng-Jie YangAbstractPlant essential oils and its chemical compositions are commonly applied in medicinal and other industries due to their broad advanced pharmacological activities. In the present study, we systematically evaluated the acaricidal activities of twelve compounds of essential oils against Psoroptes cuniculi in vitro and in vivo. In addition, to support the clinic uses, their toxicities against immortalized human keratinocytes (HaC...
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

A novel, high-welfare methodology for evaluating poultry red mite interventions in vivo
Publication date: Available online 12 February 2019Source: Veterinary ParasitologyAuthor(s): Francesca Nunn, Kathryn Bartley, Javier Palarea-Albaladejo, Giles T. Innocent, Frank Turnbull, Harry W. Wright, Alasdair J. NisbetAbstractOptimisation and use of a device for the on-hen in vivo feeding of all hematophagous stages of Dermanyssus gallinae. The sealed mesh device contains the mites and is applied to the skin of the hen’s thigh where mites can feed on the bird through a mesh which has apertures large enough to allow the mites’ mouth-parts to access to the bird but small enough to contain the mites. By optim...
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

Detection and quantification of Parascaris P-glycoprotein drug transporter expression with a novel mRNA hybridization technique
Publication date: Available online 12 February 2019Source: Veterinary ParasitologyAuthor(s): Jeba Jesudoss Chelladurai, Matthew T. BrewerAbstractMacrocyclic lactone-resistant Parascaris have been reported throughout the world. In part, the drug resistant phenotype is hypothesized to be associated with ATP-binding cassette transporters known as P-glycoproteins. In many systems, P-glycoproteins efflux drugs out of cells thereby precluding drug binding to target receptors. Parascaris may evade macrocyclic lactone-mediated death by effluxing drugs away from target receptors in the nervous system. Alternatively, P-glycoprotein ...
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

The route of Besnoitia besnoiti tachyzoites inoculation does not influence the clinical outcome of the infection in calves
Publication date: Available online 12 February 2019Source: Veterinary ParasitologyAuthor(s): C. Diezma-Díaz, I. Ferre, M. Re, A. Jiménez-Meléndez, E. Tabanera, M. González-Huecas, M. Pizarro-Díaz, D. Yanguas-Pérez, P.L. Brum, J. Blanco-Murcia, L.M. Ortega-Mora, G. Álvarez-GarcíaAbstractIn a previous attempt, an experimental model of bovine besnoitiosis was established in calves that were intravenously inoculated with different doses of Besnoitia besnoiti tachyzoites. Despite the fact that all infected calves developed the acute stage of disease, only microscopic findi...
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

Comment on the paper "Chemical analyses and anthelmintic effects of Artemisia campestris essential oil"
Publication date: Available online 12 February 2019Source: Veterinary ParasitologyAuthor(s): Emelda Ongo (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

Patent infections with Fasciola hepatica and paramphistomes (Calicophoron daubneyi) in dairy cows and their association with individual milk production and fertility parameters
Publication date: Available online 13 February 2019Source: Veterinary ParasitologyAuthor(s): Katharina May, Kerstin Brügemann, Sven König, Christina StrubeAbstractInfections with the liver fluke Fasciola hepatica may result in considerable economic losses in the dairy livestock industry worldwide. Infections have been associated with detrimental impacts on milk production and milk quality as well as reduced fertility. However, most related data rely on examinations on herd level and the rather few studies on individual cow level are based solely on antibodies as measure for F. hepatica infections. This entails th...
Source: Veterinary Parasitology - February 13, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: February 2019Source: Veterinary Parasitology, Volume 266Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 6, 2019 Category: Veterinary Research Source Type: research

Immune response against subclinical haemonchosis in Himalayan hill goats
Publication date: Available online 29 January 2019Source: Veterinary ParasitologyAuthor(s): A.K. Nehra, G.R. Gowane, A. Kuriyal, A. Chaurasiya, R. Kumar, D.B. Bhinsara, B.C. Parthasarathi, K. Bhawana, R.K. Khare, A. Prasad, D. Chandra, M. SankarAbstractHaemonchosis commonly occurs as chronic and subclinical infection in small ruminants, and understanding of immunological response against subclinical haemonchosis is of paramount importance for designing and implementing effective control strategies. The present study was designed to evaluate immunological response during subclinical haemonchosis, experimentally established ...
Source: Veterinary Parasitology - January 30, 2019 Category: Veterinary Research Source Type: research

Anticoccidial activity of novel triazine compounds in broiler chickens
The objective of present studies was to evaluate and compare the anticoccidial activity of triazine compounds in broiler chickens infected with E. tenella, E. necatrix, E. acervulina, E. maxima, and two field mixed Eimeria species. The anticoccidial efficacy was evaluated using the anticoccidial index (ACI). The results showed that Aminomizuril (AZL) and Ethanamizuril (EZL) were active metabolites of nitromezuril, which demonstrated excellent effectiveness against E. tenella, E. necatrix, E. acervulina, E. maxima, and the field Eimeria isolates in broiler chickens at a dosage of 10 mg/kg in feed. The anticoccidial activiti...
Source: Veterinary Parasitology - January 30, 2019 Category: Veterinary Research Source Type: research

Characterization of a novel cathepsin L-like protease from Taenia solium metacestodes for the immunodiagnosis of porcine cysticercosis
In conclusion, a novel cathepsin L-like cysteine protease from a T. solium metacestode was expressed successfully in Baculovirus system and was evaluated as a candidate antigen to diagnose porcine cysticercosis using the ELISA immunoassay. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 30, 2019 Category: Veterinary Research Source Type: research

First report of Neotrombicula inopinata infestation in domestic cats from Portugal
Publication date: Available online 30 January 2019Source: Veterinary ParasitologyAuthor(s): David W. Ramilo, Carla Monteiro, Marrion Carreira, Isabel Pereira da Fonseca, Luís CardosoAbstractTrombiculids parasitize a wide variety of terrestrial vertebrates, including domestic animals, throughout the world. They are parasites only during their larval stages, causing several dermatological lesions on their hosts, such as acute dermatitis, erythema, excoriation, erosion, papules, crusts and alopecia on the ear margins, face, interdigital spaces and abdomen. Neotrombicula is one of the several genera in Trombiculidae fam...
Source: Veterinary Parasitology - January 30, 2019 Category: Veterinary Research Source Type: research

IDO, COX and iNOS have an important role in the proliferation of Neospora caninum in neuron/glia co-cultures
Publication date: Available online 22 January 2019Source: Veterinary ParasitologyAuthor(s): L.B Jesus, A.B. Santos, E.E.V. Jesus, R.G.D. Santos, M.S. Grangeiro, A.B. Silva, M.R. Arruda, D.S. Argolo, A.M Pinheiro, R.S. El- Bachá, S.L. Costa, M.F.D. CostaAbstractCentral nervous system (CNS) is the main site for encystment of Neospora caninum in different animal species. In this tissue, glial cells (astrocytes and microglia) modulate responses to aggression in order to preserve homeostasis and neuronal function. Previous data showed that when primary cultures of glial cells are infected with N. caninum, they develop gl...
Source: Veterinary Parasitology - January 22, 2019 Category: Veterinary Research Source Type: research

Paralogs vs. genotypes? Variability of Babesia canis assessed by 18S rDNA and two mitochondrial markers
Publication date: Available online 18 January 2019Source: Veterinary ParasitologyAuthor(s): Kristýna Hrazdilová, Izabella Myśliwy, Joanna Hildebrand, Katarzyna Buńkowska-Gawlik, Bartłomiej Janaczyk, Agnieszka Perec-Matysiak, David ModrýAbstractCanine babesiosis caused by Babesia canis sensu stricto became an emerging disease of dogs across Europe calling for attention also in countries where it was an only rare imported disease. An easy accessibility of molecular methods and the growing amount of sequencing data led to the description of intraspecific variability in 18S rDNA sequences designated as ...
Source: Veterinary Parasitology - January 20, 2019 Category: Veterinary Research Source Type: research

Molecular data reveal cryptic speciation and host specificity in Toxascaris leonina (Nematoda: Ascarididae)
Publication date: Available online 17 January 2019Source: Veterinary ParasitologyAuthor(s): Renata Fogt-Wyrwas, Mirosława Dabert, Wojciech Jarosz, Izabela Rząd, Bogumiła Pilarczyk, Hanna Mizgajska-WiktorAbstractToxascaris leonina (Ascarididae) is a cosmopolitan and polyxenical parasite whose host are canids and felids. To date, molecular phylogenetic studies included toxascarid representatives collected only from dogs or felids, therefore the intra-species differences between T. leonina collected from different host species has not been noticed. In this paper, we test the hypothesis of cryptic speciation in the T. leoni...
Source: Veterinary Parasitology - January 18, 2019 Category: Veterinary Research Source Type: research

Towards green drugs against cestodes: Effectiveness of Pelargonium roseum and Ferula gummosa essential oils and their main component on Echinococcus granulosus protoscoleces
Publication date: Available online 17 January 2019Source: Veterinary ParasitologyAuthor(s): Mohaddeseh Abouhosseini Tabari, Mohammad Reza Youssefi, Mojtaba Nasiri, Mojtaba Hamidi, Komeil Kiani, Shohre Alian Samakkhah, Filippo MaggiAbstractCystic echinococcosis is of great public health importance. Surgery is the efficient treatment for this infection. To minimize the risk of cyst intraoperative leakage, using scolicidals is crucial. To date, any efficacious scolicidal without side effect has not been introduced. Since essential oils of Pelargonium roseum and Ferula gummosa have shown several bioactivities, we evaluated the...
Source: Veterinary Parasitology - January 17, 2019 Category: Veterinary Research Source Type: research

Survival of Taenia saginata eggs under different environmental conditions
This study aimed to assess in vitro the transmission potential of T. saginata eggs stored in different media at various temperatures, and to optimize recovery, hatching and activation methods. A total of 42 freshwater and 42 silt samples were spiked with 100 μl of a T. saginata egg suspension, of which half were stored at 5 °C and the other half at 20 °C. Additionally, 5 silt and 35 tap water control samples were included. Eggs were obtained from gravid proglottids passed by a human carrier following treatment. The duration of the experiment was 6 months, with three sampling time points at 2...
Source: Veterinary Parasitology - January 15, 2019 Category: Veterinary Research Source Type: research

Pharmacokinetic profile and anthelmintic efficacy of moxidectin administered by different doses and routes to feedlot calves
This study was divided into two separate studies, the “Pharmacokinetic (PK) study” and the “Efficacy study”. The “PK study” involved 24 calves free of gastrointestinal nematodes (GIN), which were allocated into 4 groups (n = 6) and treated with MXD by either the SC or the IR route at the therapeutic (MXDSC0.2, MXDIR0.2, respectively) or at fivefold the therapeutic dose (MXDSC1.0, MXDIR1.0, respectively). Blood samples were collected from 3 h up to 14 days post-treatment. MXD concentrations in plasma samples were analyzed by HPLC. The “Efficacy study” incl...
Source: Veterinary Parasitology - January 12, 2019 Category: Veterinary Research Source Type: research

Development of an antigen ELISA using monoclonal antibodies against recombinant VSG for the detection of active infections of Trypanosoma evansi in animals
Publication date: Available online 11 January 2019Source: Veterinary ParasitologyAuthor(s): P.P. Sengupta, G.R. Rudramurthy, M. Ligi, S.S. Jacob, H. Rahman, P. RoyAbstractTrypanosoma evansi, a haemo-flagellated protozoan parasite causes chronic wasting disease in a wide range of animals. For its diagnosis, blood smear examination is useful in clinical cases for direct identification of the parasite but in latent infection the carrier animals are difficult to screen out by conventional blood smear test. Harboring low level of parasites and showing no symptom, the carrier animals for surra can act as a source of infection. T...
Source: Veterinary Parasitology - January 12, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: January 2019Source: Veterinary Parasitology, Volume 265Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 11, 2019 Category: Veterinary Research Source Type: research

Comparison of multiplexed-tandem real-time PCR panel with reference real-time PCR molecular diagnostic assays for detection of Giardia intestinalis and Tritrichomonas foetus in cats
In conclusion, the MT-PCR Small Animal Diarrhoea panel had a good and poor performance against reference assays for G. intestinalis and T. foetus, respectively. The assay is suitable for detection and differential diagnosis of G. intestinalis and moderate to high burdens of T. foetus in small animal clinical practice.Graphical abstract (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 10, 2019 Category: Veterinary Research Source Type: research