Infection of Lutzomyia longipalpis in cats infected with Leishmania infantum
This study evaluated the infectiousness of to phlebotomine sand flies cats infected with L. infantum. A total of 12 cats with infection by L. infantum from the city of Teresina, Piauí, Brazil, were included in the study. Cats were diagnosed by direct visualization of the parasite. Laboratory-bred insects, free from infection by Leishmania spp. were offered a blood meal for 60 min on cats infected with L. infantum. On the fifth and sixth day after the blood meal, flies were dissected to assess promastigote forms of the parasite in the digestive system. Eight cats (67%) were able to infect the vectors. The freq...
Source: Veterinary Parasitology - February 13, 2020 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: February 2020Source: Veterinary Parasitology, Volume 278Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 10, 2020 Category: Veterinary Research Source Type: research

Is there competition between Haemonchus contortus and Haemonchus placei in a pasture grazed by only sheep?
In conclusion, hybridization between H. contortus and H. placei can occur in the field during coinfection. It was demonstrated that H. placei established successfully in artificially infected worm-free sheep; however, with concomitant natural reinfection with H. contortus, the H. placei population showed a rapid decrease and was eliminated within a few months in an environment without cattle. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 8, 2020 Category: Veterinary Research Source Type: research

Diversity of Trichinella species in relation to the host species and geographical location
Publication date: Available online 5 February 2020Source: Veterinary ParasitologyAuthor(s): Ewa Bilska-Zając, Mirosław Różycki, Katarzyna Grądziel-Krukowska, Aneta Bełcik, Iwona Mizak, Jacek Karamon, Jacek Sroka, Jolanta Zdybel, Tomasz CencekAbstractTrichinella nematodes still circulate in various hosts in both domestic and sylvatic environments. Recently, in Europe, the transmission of Trichinella spp. to humans has been attributed more to wild animals than to domestic animals. However, domestic animals could still be a source of human infections in some regions. Therefore, our aim was to determine the species...
Source: Veterinary Parasitology - February 7, 2020 Category: Veterinary Research Source Type: research

Haemonchus contortus transthyretin domain - containing protein (HcTTR): a promising vaccine candidate against Haemonchus contortus infection
Publication date: Available online 5 February 2020Source: Veterinary ParasitologyAuthor(s): Xiaowei Tian, Mingmin Lu, Caiwen Jia, Yongqian Bu, Kalibixiati Aimulajiang, Yang Zhang, Charles Li, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui LiAbstractHaemonchus contortus transthyretin domain-containing protein (HcTTR) with 136 amino acids belongs to a transthyretin-like (TTL) family member. In our previous study, it was reported that HcTTR was a novel antagonist of the goat cytokine Interleukin 4 (IL-4), and was involved in the regulation of host immune responses, implying that it might be applied for therapeutic strategies o...
Source: Veterinary Parasitology - February 5, 2020 Category: Veterinary Research Source Type: research

Ctenocephalides felis (cat flea) infestation in neonatal dairy calves managed with deltamethrin pour-on in Australia
We report successful improvement of welfare and reduction of flea counts post application of pour-on deltamethrin on the property. In the absence of registered flea product for cattle in Australia, deltamethrin pour-on product is a suitable option, because of its registration for control of lice and flies on cattle. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - February 1, 2020 Category: Veterinary Research Source Type: research

Molecular epidemiology and associated risk factors of Anaplasma marginale and Theileria annulata in cattle from North-western Pakistan
Publication date: Available online 31 January 2020Source: Veterinary ParasitologyAuthor(s): Jehan Zeb, Sumaira Shams, Israr Ud Din, Sultan Ayaz, Adil Khan, Nasreen Adil, Hamidullah Amin, Munsif Ali Khan, Haytham SenbillABSTRACTTicks and tick-borne pathogens are one of the major threats to livestock production worldwide. The aim of present study is to specify the molecular epidemiology and its associated risk factors of two well-distributed tick-borne pathogens Anaplasma marginale and Theileria annulata in cattle population from North-western Pakistan. Blood samples were Collected from 60 (32.6%) local breed cattle, 101 (54...
Source: Veterinary Parasitology - February 1, 2020 Category: Veterinary Research Source Type: research

Scientific achievements of the last 60 years: From a single to a multispecies concept of the genus Trichinella
Publication date: Available online 30 January 2020Source: Veterinary ParasitologyAuthor(s): Edoardo PozioAbstractThe scientific basis that led to the development of a multispecies concept within the Trichinella genus originated in the 1950s, when scientists began reporting an increasing number of host-specific peculiarities among different geographic isolates. This led to speculation that important geographic variability existed within Trichinella spiralis, the only species in the genus at that time. Comparative infection results sparked great interest among investigators and led to similar studies using various geographic...
Source: Veterinary Parasitology - February 1, 2020 Category: Veterinary Research Source Type: research

Caracterization of glutathione S-transferase of Dermacantor marginatus and effect of the recombinant antigen as a potential anti-tick vaccine
Publication date: Available online 30 January 2020Source: Veterinary ParasitologyAuthor(s): Huercha, Ruiqi Song, Min Li, Xinli Fan, Zhengxiang Hu, Wu Lijiang, Yongchang Li, Wei Zhang, Yang Zhang, Yuhui Ma, Chahan BayinAbstractDermacentor marginatus is one of the main tick species in northwestern China, and is a vector of various tick-borne pathogens. Tick control method largely depends on chemical agents, but the disadvantages of using such approach would cause environmental damage and the risk of developing tick resistance to acaricides. Vaccination of tick protective antigen is an eco-friendly approach which is an altern...
Source: Veterinary Parasitology - January 30, 2020 Category: Veterinary Research Source Type: research

Wild ruminants as reservoirs of domestic livestock gastrointestinal nematodes
Publication date: Available online 28 January 2020Source: Veterinary ParasitologyAuthor(s): Carly D. Barone, Janneke Wit, Eric P. Hoberg, John S. Gilleard, Dante S. ZarlengaAbstractGastrointestinal nematode (GIN) infections in cattle cause appetite suppression which leads to poor feed conversion, reduced weight gain and reduced milk production. Overuse and exclusive reliance on anthelmintic drugs has resulted in widespread resistance in many parasitic nematode species infecting livestock making control increasingly difficult. Wild ruminants are competent hosts of a number of nematode species that typically infect and are b...
Source: Veterinary Parasitology - January 29, 2020 Category: Veterinary Research Source Type: research

In vitro acaricidal activity of essential oil and alcoholic extract of Trachyspermum ammi against Dermanyssus gallinae
This study aimed to assess in vitro acaricidal activity of essential oil (EO) and alcoholic extracts (AE) of Ajowan against D. gallinae. Using contact and spraying bioassays, different doses of EO and AE were tested. Cypermethrin and thymol (positive controls), and ethanol and distilled water (negative controls) were also tested. The results indicated that effects were method and dose dependent. Statistical analysis showed a highly significant difference for contact and spraying bioassays and various doses of EO and AE (p
Source: Veterinary Parasitology - January 27, 2020 Category: Veterinary Research Source Type: research

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of the E198A SNP in the isotype-1 β-tubulin gene of Haemonchus contortus populations in China
In conclusion, the developed LAMP method has high specificity and good reproducibility for screening the E198A SNP of isotype-1 β-tubulin gene of H. contortus of field samples without using sophisticated equipment, providing useful technique for the rapid detection and thus prevention and control of benzimidazole resistant H. contortus infections. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 24, 2020 Category: Veterinary Research Source Type: research

Integrating the control of helminths in dairy cattle: deworming, rotational grazing and nutritional pellets with parasiticide fungi
Publication date: Available online 22 January 2020Source: Veterinary ParasitologyAuthor(s): Mathilde Voinot, Cristiana Cazapal-Monteiro, José Ángel Hernández, Antonio Miguel Palomero, Fabián Leonardo Arroyo, Jaime Sanchís, José Pedreira, Rita Sánchez-Andrade, Adolfo Paz-Silva, María Sol AriasAbstractThirty-two Friesian cattle under a leaders/followers four-day rotation and passing eggs of trematodes and gastrointestinal nematodes (GIN) were studied in two trials for the integrated control of these helminths over two years. In the first trial, the effect of rotational ...
Source: Veterinary Parasitology - January 23, 2020 Category: Veterinary Research Source Type: research

The effect of maternal Strongyloides venezuelensis infection on mice offspring susceptibility and immune response
Publication date: Available online 22 January 2020Source: Veterinary ParasitologyAuthor(s): Fernanda S. Costa, Vanessa Fernandes Rodrigues, Michelle Carvalho de Rezende, Jailza Lima Rodrigues, Paulo M.Z. Coelho, Deborah Negrão-CorrêaAbstractSpecies of Strongyloides infect a wide range of hosts worldwide. Due to their complex life cycle, it is hard to control the transmission of these parasites. Several species show evidence of vertical transmission; however, the impact of this transmission route on the susceptibility of the offspring has been poorly investigated. Herein, we used Strongyloides venezuelensis inf...
Source: Veterinary Parasitology - January 23, 2020 Category: Veterinary Research Source Type: research

Endemic instability of ovine babesiosis in Turkey: a country-wide sero-epidemiological study
This study assessed whether the endemic status of this disease was stable or instable, which is important for disease control efforts. For this aim, 4115 sheep blood samples were collected from 81 cities in the seven geographical regions of Turkey. The diagnosis of Babesia ovis was made using microscopic and serological techniques. Thin blood smears were prepared from anticoagulated venous blood. Serum samples were screened for specific antibodies using an enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT). Recombinant Babesia ovis secreted antigen 1 (rBoSA1) was used in the ELISA. The ...
Source: Veterinary Parasitology - January 21, 2020 Category: Veterinary Research Source Type: research

Multilocus approach reveals discordant molecular markers and corridors for gene flow between North African populations of Fasciola hepatica
Publication date: Available online 21 January 2020Source: Veterinary ParasitologyAuthor(s): Nabil Amor, Sarra Farjallah, Paolo Merella, Abdulaziz N Alagaili, Osama B. MohammedAbstractFasciolosis is a foodborne trematodosis characterised by a worldwide distribution. Various approaches have been developed for the study of the causative agents of this parasitic infection: Fasciola hepatica, Fasciola gigantica and the aspermic intermediated forms (hybrid and introgressed). In the present study, novel and common molecular markers (pepck and pold, ITS, CO1, ND1 and CO1-trnT-rrnL) were used to characterise Fasciola flukes from th...
Source: Veterinary Parasitology - January 21, 2020 Category: Veterinary Research Source Type: research

ATTRACT OR REPEL Amblyomma sculptum TICKS: SCREENING OF SEMIOCHEMICALS
Publication date: Available online 21 January 2020Source: Veterinary ParasitologyAuthor(s): Lorena Lopes Ferreira, Jaires Gomes de Oliveira Filho, Fernanda de Oliveira Silva, Ana Livia Lacerda Ferraz, Gabriel Moura MascarinAbstractAmblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitized hosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages such as high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick att...
Source: Veterinary Parasitology - January 21, 2020 Category: Veterinary Research Source Type: research

Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and potentiates the effect of monepantel in the parasitic nematode Ascaris suum
Publication date: Available online 20 January 2020Source: Veterinary ParasitologyAuthor(s): Djordje S. Marjanović, Nemanja Zdravković, Mirjana Milovanović, Jelena Nedeljković Trailović, Alan P. Robertson, Zoran Todorović, Saša M. TrailovićAbstractThe neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have exami...
Source: Veterinary Parasitology - January 20, 2020 Category: Veterinary Research Source Type: research

Which larvae are they? Use of single larva for the molecular confirmation of Cooperia pectinata and Cooperia punctata in Australian cattle
Publication date: Available online 18 January 2020Source: Veterinary ParasitologyAuthor(s): Emily Kate Francis, Janina McKay-Demeler, Nichola Eliza Davies Calvani, Denise McDonell, Jan ŠlapetaAbstractIn Australia, Cooperia spp. are often overshadowed by parasites believed to be more pathogenic production-limiting nematodes. A rise in anthelmintic resistance and reports of reduced growth rates attributed to infection with Cooperia spp. in Europe increases the need to be able to monitor the presence of C. pectinata, C. punctata and C. oncophora in Australian cattle. Here, we present the first molecular confirmation of...
Source: Veterinary Parasitology - January 19, 2020 Category: Veterinary Research Source Type: research

In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4
Publication date: Available online 17 January 2020Source: Veterinary ParasitologyAuthor(s): Pengfei Zhao, Yuncan Li, Yanqin Zhou, Junlong Zhao, Rui FangAbstractEimeria tenella, belonging to protozoon, is the causative agent of cecal coccidiosis in chicken and causes enormous impacts for poultry industry. The surface antigens of apicomplexan parasites function as attachment and invasion in host-parasite interaction. Meanwhile, host immune response is triggered as a result of parasitic invasion. Immunogenicity and potency as a vaccinal candidate antigen of E. tenella surface antigen 4 (EtSAG4) have been unknown. Therefore, a...
Source: Veterinary Parasitology - January 18, 2020 Category: Veterinary Research Source Type: research

Modifications of Histones in Parasites as drug targets
Publication date: Available online 15 January 2020Source: Veterinary ParasitologyAuthor(s): Mohsin Nawaz, Irfan Malik, Mudassar Hameed, Zulfiqar Hussain Kuthu, Jinlin ZhouAbstractPost-translational modifications of histones and histone modifying enzymes play important roles in gene regulations and other physiological processes in parasites. Inhibitors of such modifying enzymes could be useful as novel therapeutics against parasitic diseases or as chemical probes for investigation of epigenetics. Development of parasitic histone modulators has got rapid expansion in the last few years. A number of highly potent and selectiv...
Source: Veterinary Parasitology - January 16, 2020 Category: Veterinary Research Source Type: research

Set up of an in vitro model to study early host-parasite interactions between newly excysted juveniles of Fasciola hepatica and host intestinal cells using a quantitative proteomics approach
The objective of the present work is to characterize the proteomic changes occurring in both the parasite and the host, through the development of a novel in vitro model, to shed light on the molecular pathways of communication between the newly excysted juveniles (NEJ) from F. hepatica and the host’s intestinal epithelium. For this, in vitro excystation of F. hepatica metacercariae was carried out and NEJ were obtained. Additionally, optimal conditions of growth and expansion of mouse primary small intestinal epithelial cells (MPSIEC) in culture were fine-tuned. Tegumentary and somatic parasite antigens (NEJ-Teg and...
Source: Veterinary Parasitology - January 16, 2020 Category: Veterinary Research Source Type: research

Optimal age of Trichostrongylus colubriformis larvae (L3) for the in vitro larval exsheathment inhibition test under tropical conditions
This study identified the optimal age of Trichostrongylus colubriformis larvae (L3) under tropical conditions for the in vitro evaluation of plant extracts using the larval exsheathment inhibition test (LEIT). Two T. colubriformis isolates with different anthelmintic (AH) susceptibility status were used for this study. The L3 of both isolates were maintained on refrigeration (4-5 °C) until use. For the LEIT, the isolates were tested every week during 16 weeks, using a stock solution of Acacia pennatula acetone:water extract at different dilutions (80 to 1200 μg/mL). Respective positive controls (levami...
Source: Veterinary Parasitology - January 10, 2020 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: January 2020Source: Veterinary Parasitology, Volume 277Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 10, 2020 Category: Veterinary Research Source Type: research

Determination of the prevalence and intensity of Fasciola hepatica infection in dairy cattle from six irrigation regions of Victoria, South-eastern Australia, further identifying significant triclabendazole resistance on three properties
This study has confirmed that F. hepatica is endemic in several dairy regions in Victoria: triclabendazole resistance may be contributing to the high prevalence in some herds. From our analysis, we estimate that the state-wide economic loss associated with fasciolosis is in the order of AUD 129 million (range AUD 38-193 million) per year or about AUD 50,000 (range AUD 15,000-75,000) per herd per year.Graphical abstract (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - January 1, 2020 Category: Veterinary Research Source Type: research

Fasciola hepatica seroprevalence in Northern German dairy herds and associations with milk production parameters and milk ketone bodies
Publication date: Available online 27 December 2019Source: Veterinary ParasitologyAuthor(s): Katharina May, Ernst Bohlsen, Sven König, Christina StrubeAbstractInfections with the liver fluke Fasciola hepatica remain a serious problem in dairy herds causing significant production losses. In sheep, a strong relationship between F. hepatica infections and an increase in serum ketone bodies due to reduced feed intake and liver damage was demonstrated. We hypothesized that F. hepatica infections might contribute to an increase in milk ketone bodies in dairy herds. Thus, the objective of the study was to estimate the associ...
Source: Veterinary Parasitology - December 29, 2019 Category: Veterinary Research Source Type: research

Assessment of targeted selective treatment criteria to control subclinical gastrointestinal nematode infections on sheep farms
Publication date: Available online 27 December 2019Source: Veterinary ParasitologyAuthor(s): Carlos Calvete, José M. González, Luis M. Ferrer, Juan J. Ramos, Delia Lacasta, Ignacio Delgado, Joaquín UriarteAbstractControl of sheep gastrointestinal nematode (GIN) infections mostly relies on the use of anthelmintics. Refugia-based control strategies as targeted selective treatments (TST) can delay anthelmintic resistance development, but the optimal decision criteria for selecting individuals to be treated in subclinical infection scenarios remain unclear. The present study aimed to evaluate the suitabili...
Source: Veterinary Parasitology - December 27, 2019 Category: Veterinary Research Source Type: research

Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
Publication date: Available online 23 December 2019Source: Veterinary ParasitologyAuthor(s): Héctor Gabriel Avila, Cecilia Mozzoni, Marcos David Trangoni, Silvio Lorenzo Pedro Cravero, Verónica Mirtha Pérez, Federico Valenzuela, María Laura Gertiser, Marcos Javier Butti, Laura Kamenetzky, Oscar Jensen, Mara Cecilia RosenzvitAbstractCystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (...
Source: Veterinary Parasitology - December 25, 2019 Category: Veterinary Research Source Type: research

Development and field evaluation of a species-specific mt-COI targeted SYBR-Green Real Time PCR for detection and quantification of Haemonchus contortus in cattle in Turkey
Publication date: Available online 23 December 2019Source: Veterinary ParasitologyAuthor(s): Onder Duzlu, Alparslan Yildirim, Gamze Yetismis, Zuhal Onder, Emrah Simsek, Arif Ciloglu, Gozde Sahingoz Demirpolat, Abdullah InciAbstractHaemonchus contortus is one of the most important gastrointestinal nematodes (GINs) infecting sheep, goats, and cattle worldwide. We developed a SYBR Green real-time PCR (qPCR) assay for detection and quantification of H. contortus by using specific primers based on a conserved region of the mitochondrial cytochrome oxidase subunit I (mt-COI) gene, and evaluated this technique in the detection of...
Source: Veterinary Parasitology - December 25, 2019 Category: Veterinary Research Source Type: research

Evaluation of serum symmetric dimethylarginine as a biomarker of kidney disease in canine leishmaniosis due to Leishmania infantum
The objectives of this study were to investigate sSDMA concentrations in dogs with CanL, with and without azotemia, and to establish any potential association with the prevalence and severity of proteinuria, with the prevalence of decreased urine specific gravity and with the LeishVet clinical stages of CanL. Serum samples from 68 dogs with CanL (50 nonazotemic and 18 azotemic) and 17 healthy dogs were retrospectively examined. Increased sSDMA was documented in 26% of dogs with CanL without azotemia and in 83.3% of dogs with azotemia. Serum SDMA was significantly higher in azotemic compared to nonazotemic dogs and was asso...
Source: Veterinary Parasitology - December 19, 2019 Category: Veterinary Research Source Type: research

Prevalence of Trypanosoma cruzi infection and associated histologic findings in domestic cats (Felis catus)
Publication date: Available online 16 December 2019Source: Veterinary ParasitologyAuthor(s): Italo B. Zecca, Carolyn L. Hodo, Sarah Slack, Lisa Auckland, Sandy Rodgers, Keswick C. Killets, Ashley B. Saunders, Sarah A. HamerAbstractTrypanosoma cruzi is a zoonotic protozoan parasite mainly transmitted by triatomines and is known to infect a wide range of mammals. South Texas is a hotspot for triatomines, T. cruzi-infected dogs and wildlife, and local transmission to humans also occurs. However, little is known about the infection of domestic cats (Felis catus) in the United States. Given the role cats play in the ecology of ...
Source: Veterinary Parasitology - December 17, 2019 Category: Veterinary Research Source Type: research

Assay Methods for in Vitro and in Vivo Anti-Babesia Drug Efficacy Testing: Current Progress, Outlook, and Challenges
Publication date: Available online 13 December 2019Source: Veterinary ParasitologyAuthor(s): Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Medhat Nassif, Juan Mosqueda, Xuenan Xuan, Ikuo IgarashiAbstractAbsence of an effective high-throughput drug-screening system for Babesia parasites is considered one of the main causes for the presence of a wide gap in the treatment of animal babesiosis when compared with other hemoprotozoan diseases, such as malaria. Recently, a simple, accurate, and automatic fluorescence assay was established for large-scale anti-Babesia (B. bovis, B. bigemina, B. divergens, B. caballi and T. equi...
Source: Veterinary Parasitology - December 14, 2019 Category: Veterinary Research Source Type: research

Added value of IgM detection and low avidity index as markers of acute bovine besnoitiosis
In this study, a novel ELISA to detect specific anti-Besnoitia besnoiti IgM antibodies was developed. The usefulness of this tool together with an avidity ELISA were studied with a well-coded sera panel from experimentally and naturally infected cattle. First, the kinetics of specific IgM levels were determined in experimentally infected calves during the acute and chronic infection. Next, IgM levels were determined in naturally infected cattle with either acute or chronic infection. Finally, the IgG avidity index was monitored in both experimentally and naturally infected cattle.Specific IgM antibodies were detected prior...
Source: Veterinary Parasitology - December 13, 2019 Category: Veterinary Research Source Type: research

Pharmacokinetic-pharmacodynamic assessment of the ivermectin and abamectin nematodicidal interaction in cattle
Publication date: Available online 10 December 2019Source: Veterinary ParasitologyAuthor(s): M. Ballent, C. Canton, P. Dominguez, G. Bernat, C. Lanusse, G. Virkel, A. LifschitzAbstractIn a context of nematodicidal resistance, anthelmintic combinations have emerged as a reliable pharmacological strategy to control gastrointestinal nematodes in grazing systems of livestock production. The current work evaluated the potential drug-drug interactions following the coadministration of two macrocyclic lactones (ML) ivermectin (IVM) and abamectin (ABM) to parasitized cattle using a pharmacokinetic/pharmacodynamic (PK/PD) approach....
Source: Veterinary Parasitology - December 10, 2019 Category: Veterinary Research Source Type: research

Characterization of acaricide resistance in Rhipicephalus microplus populations infesting cattle in northeastern India and assessment of local plant extracts for tick management
Publication date: Available online 5 December 2019Source: Veterinary ParasitologyAuthor(s): Deepak Upadhaya, Bhanu Kumar, Sachin Kumar, Anil Kumar Sharma, Ashutosh Fular, Nisha Bisht, Sharad Srivastava, Rashmi Rekha Boruah, Gaurav Nagar, Mukesh Shakya, Tankeswar Nath, Shoma Paul Nandi, Karivaradharaja S, Srikant GhoshAbstractIn the present study, the tick isolates were collected from Assam state, of northeastern region (NER) and characterized using in vitro bioassay, biochemical and molecular assays. Comparing LC50 value of susceptible IVRI-I and larvae of field isolates, revealed that RF against deltamethrin was highest f...
Source: Veterinary Parasitology - December 6, 2019 Category: Veterinary Research Source Type: research

Beneficial effects of dietary supplementation of Bacillus strains on growth performance and gut health in chickens with mixed coccidiosis infection
In conclusion, the results of this study are promising and indicate the many beneficial effects of DFM-supplementation in poultry diets to reduce the negative consequences of enteric diseases and to decrease economic losses imposed by coccidia infection in chickens. (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - December 4, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: December 2019Source: Veterinary Parasitology, Volume 276Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - December 4, 2019 Category: Veterinary Research Source Type: research

Retrospective survey of parasitism identified in feces of client-owned cats in North America from 2007 through 2018
Publication date: Available online 3 December 2019Source: Veterinary ParasitologyAuthor(s): Yoko Nagamori, Mark E. Payton, Emily Looper, Hadley Apple, Eileen M. JohnsonAbstractOwnership of domestic cats in North America has been on the increase; however, there are only a few surveys conducted on the prevalence of parasitism in client-owned cats over years. Our study objective was to evaluate parasite prevalence through statistical analysis of fecal examination results for client-owned cats on samples submitted to the veterinary parasitology diagnostic laboratory of Oklahoma State University over a 12-year period. All resul...
Source: Veterinary Parasitology - December 4, 2019 Category: Veterinary Research Source Type: research

Menace of acaricide resistance in cattle tick, Rhipicephalus microplus in India: Status and possible mitigation strategies
Publication date: Available online 26 November 2019Source: Veterinary ParasitologyAuthor(s): Rinesh Kumar, Anil Kumar Sharma, Srikant GhoshAbstractLivestock in general and dairy sector in particular plays a vital role in the Indian economy and in the socio-economic development of millions of people. Rhipicephalus microplus, a one-host and the most prevalent cattle tick in various agro climatic zones of India. This tick species is responsible for severe economic losses to livestock owners both through the direct effects of blood sucking, injection of toxins and indirectly by transmitting pathogens. Due to inadvertent and in...
Source: Veterinary Parasitology - November 27, 2019 Category: Veterinary Research Source Type: research

Review of the Eprinomectin effective doses required for dairy goats: where do we go from here?
Publication date: Available online 22 November 2019Source: Veterinary ParasitologyAuthor(s): Antoine Rostang, Jacques Devos, Christophe ChartierAbstractEprinomectin (EPM) has been recently granted a marketing authorisation in the European Union for use in goats, with a zero-day milk withdrawal period. Considering the high prevalence of benzimidazole resistance worldwide and the economic implications of managing milk residues, EPM may today be considered the main (or even the only) affordable treatment option, at least in dairy goats in the EU. However, the chosen dose (1 mg/kg) seems to be suboptimal, especially for...
Source: Veterinary Parasitology - November 23, 2019 Category: Veterinary Research Source Type: research

The prevalence of Dirofilaria immitis and D. repens in the Old World
Publication date: Available online 21 November 2019Source: Veterinary ParasitologyAuthor(s): Claudio Genchi, Laura Helen KramerAbstractDirofilaria immitis and D. repens are endemic throughout Europe and southern eastern regions of Asia and reported with increasing frequency in Africa. Nevertheless, the increased awareness of veterinary practitioners, even in countries where the prevalence is low, has led to a decrease D. immitis prevalence in dogs, especially in previously endemic/hyper-endemic areas. Prevalence has significantly increased, however, in areas where heartworm has apparently spread more recently, such as Cent...
Source: Veterinary Parasitology - November 22, 2019 Category: Veterinary Research Source Type: research

Combined control evaluation for Neospora caninum infection in dairy: Economic point of view coupled with population dynamics
Publication date: Available online 21 November 2019Source: Veterinary ParasitologyAuthor(s): Yue Liu, Michael P. Reichel, Wing-Cheong LoAbstractNeospora caninum infection is regarded as one of the most important infectious causes of abortion in dairy cattle. To intervene in its spread, four potential controls including test-and-cull, medication, vaccination, and selective breeding are considered and assessed in this study. The cost of each control, together with the inevitable annual loss due to population dynamics, is adopted as an assessment criterion from an economic point of view. By performing simulation and sensitivi...
Source: Veterinary Parasitology - November 21, 2019 Category: Veterinary Research Source Type: research

A time-resolved fluorescence immunoassay for the detection of anti-Neospora caninum antibodies in sheep
Publication date: Available online 19 November 2019Source: Veterinary ParasitologyAuthor(s): Ana Huertas-López, Carlos Martínez-Carrasco, José Joaquín Cerón, Roberto Sánchez-Sánchez, Ángela Vázquez-Calvo, Gema Álvarez-García, Silvia Martínez-SubielaAbstractNeospora caninum is a protozoan parasite (Phylum Apicomplexa) that has been recently suggested as a relevant cause of reproductive disorders in small ruminants. The aim of the present study is to develop and validate a new serological test based on time resolved fluorescency using N. cani...
Source: Veterinary Parasitology - November 19, 2019 Category: Veterinary Research Source Type: research

Eukaryotic elongation factor 2 is involved in the anticoccidial action of diclazuril in the second-generation merozoites of Eimeria tenella
This study aims to explore the exact mechanisms underlying diclazuril inhibition in second-generation merozoites of E. tenella. The eEF2 cDNA of the second-generation merozoites of E. tenella (EtEF2) was cloned by reverse transcriptase polymerase chain reaction and rapid amplification of cDNA ends. Diclazuril-induced expression profiles of EtEF2 were also analyzed. The cloned full-length cDNA (2893 bp) of the EtEF2 nucleotide sequence encompassed a 2499 bp open reading frame (ORF) that encoded a polypeptide of 832 residues with an estimated molecular mass of 93.12 kDa and a theoretical isoelectric point of 5.99. The...
Source: Veterinary Parasitology - November 18, 2019 Category: Veterinary Research Source Type: research

Inducible Nitric Oxide Synthase is required for parasite restriction and inflammatory modulation during Neospora caninum infection
Publication date: Available online 16 November 2019Source: Veterinary ParasitologyAuthor(s): Patrício da Silva Cardoso Barros, Caroline Martins Mota, Vanessa dos Santos Miranda, Flávia Batista Ferreira, Eliézer Lucas Pires Ramos, Silas Silva Santana, Lourenço Faria Costa, Ana Cláudia Arantes Marques Pajuaba, José Roberto Mineo, Tiago Wilson Patriarca MineoAbstractNeospora caninum infection is an important cause of neuromuscular disease in dogs and abortion in cattle, leading to significant economic losses in beef and dairy industries. The protective immunity against apicomplexan pa...
Source: Veterinary Parasitology - November 18, 2019 Category: Veterinary Research Source Type: research

Combination of thymol and eugenol for the control of Rhipicephalus sanguineus sensu lato: Evaluation of synergism on immature stages and formulation development
Publication date: Available online 16 November 2019Source: Veterinary ParasitologyAuthor(s): Letícia Coelho, Luiza Gabriella Ferreira de Paula, Susy das Graças Alecrim Alves, Aparecida Lorrany Nunes Sampaio, Giovana Pereira Bezerra, Fernanda Maria Pinto Vilela, Renata da Silva Matos, Viviane Zeringóta, Lígia Miranda Ferreira Borges, Caio MonteiroAbstractThe aim of this study was to investigate whether the combination of thymol with eugenol has a synergistic effect on the immature life stages of Rhipicephalus sanguineus sensu lato (s.l.), to evaluate the cost-benefit ratio of using these compound...
Source: Veterinary Parasitology - November 16, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: November 2019Source: Veterinary Parasitology, Volume 275Author(s): (Source: Veterinary Parasitology)
Source: Veterinary Parasitology - November 15, 2019 Category: Veterinary Research Source Type: research

Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis
Publication date: Available online 12 November 2019Source: Veterinary ParasitologyAuthor(s): Pornanan Kueakhai, Kulathida Chaithirayanon, Athit Chaiwichien, Tepparit Samrit, Supawadee Osotprasit, Phawiya Suksomboon, Wipaphorn Jaikua, Prasert Sobhon, Narin ChangklungmoaAbstractGlutathione peroxidases (GPx), major antioxidant enzymes, secreted by Fasciola spp., are important for the parasite evasion and protection against the host’s immune responses. In the present study, a monoclonal antibody (MoAb) against recombinant F. gigantica glutathione peroxidase (rFgGPx) was produced by hybridoma technique using spleen cells ...
Source: Veterinary Parasitology - November 13, 2019 Category: Veterinary Research Source Type: research

Efficacy of sarolaner on the treatment of myiasis caused by Cochliomyia hominivorax (Diptera: Calliphoridae) in dogs
Publication date: Available online 12 November 2019Source: Veterinary ParasitologyAuthor(s): Priscila Cardim de Oliveira, Gabriela Pereira Salça de Almeida, Jaime Dias Cardoso, Renan Bernardes Tavares, Júlio Israel Fernandes, Thaís Ribeiro Correia Azevedo, Guilherme Gomes Verocai, Fabio Barbour ScottABSTRACTThe aim of this study was to evaluate the larval expulsion, larvicidal effect and overall efficacy of sarolaner on the treatment of myiasis caused by New World screwworm Cochliomyia hominivorax in naturally infested dogs. Eight Beagle dogs received a single dose of saroalner 40 mg, with dosag...
Source: Veterinary Parasitology - November 13, 2019 Category: Veterinary Research Source Type: research

A longitudinal study of the effect of Theileria orientalis Ikeda type infection on three New Zealand dairy farms naturally infected at pasture
Publication date: Available online 12 November 2019Source: Veterinary ParasitologyAuthor(s): KE Lawrence, K. Gedye, WE PomroyAbstractThe aims of this study were to monitor the change in Theileria orientalis Ikeda type infection intensity, haematocrit, milk production and reproduction on three New Zealand spring calving dairy herds, over the 2014 to 2015 milking season.Three spring calving dairy farms, A, B and C, from high risk (endemically stable), low risk (endemically unstable), and zero risk (disease-free) tick areas respectively were followed through the 2014 to 2015 milking season. On Farms, A and B, 100 cows were ra...
Source: Veterinary Parasitology - November 13, 2019 Category: Veterinary Research Source Type: research