Cancers, Vol. 12, Pages 239: In-Depth Mapping of the Urinary N-Glycoproteome: Distinct Signatures of ccRCC-related Progression

Cancers, Vol. 12, Pages 239: In-Depth Mapping of the Urinary N-Glycoproteome: Distinct Signatures of ccRCC-related Progression Cancers doi: 10.3390/cancers12010239 Authors: Lucia Santorelli Giulia Capitoli Clizia Chinello Isabella Piga Francesca Clerici Vanna Denti Andrew Smith Angelica Grasso Francesca Raimondo Marco Grasso Fulvio Magni Protein N-glycosylation is one of the most important post-translational modifications and is involved in many biological processes, with aberrant changes in protein N-glycosylation patterns being closely associated with several diseases, including the progression and spreading of tumours. In light of this, identifying these aberrant protein glycoforms in tumours could be useful for understanding the molecular mechanism of this multifactorial disease, developing specific biomarkers and finding novel therapeutic targets. We investigated the urinary N-glycoproteome of clear cell renal cell carcinoma (ccRCC) patients at different stages (n = 15 at pT1 and n = 15 at pT3), and of non-ccRCC subjects (n = 15), using an N-glyco-FASP-based method. Using label-free nLC-ESI MS/MS, we identified and quantified several N-glycoproteins with altered expression and abnormal changes affecting the occupancy of the glycosylation site in the urine of RCC patients compared to control. In particular, nine of them had a specific trend that was directly related to the stage progression: CD97, COCH and P3IP1 were up-expressed whilst APOB, F...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research