MicroRNA-145 suppresses gastric cancer progression by targeting Hu-Antigen R.

MicroRNA-145 suppresses gastric cancer progression by targeting Hu-Antigen R. Am J Physiol Cell Physiol. 2020 Jan 15;: Authors: Li Q, Tong D, Guo C, Wu F, Li F, Wang X, Jiang Q, Wei Y, Liu L, Ni L, Guo B, Huang C Abstract Hu-Antigen R (HuR) is involved in the carcinogenesis and progression of multiple types of cancer. However, its precise role in gastric cancer (GC) and the relevant molecular mechanism remain largely unclear. In the present study, we found that HuR expression level was higher in GC tissues and cell lines than in adjacent normal tissues and normal gastric epithelial cell lines, and this elevated expression was found has a significantly association with the lymph node metastasis. Moreover, Silencing HuR with RNA interference inhibited cell viability and induced cell apoptosis through the apoptosis related regulators (Bcl-2 and Bax) in GC cells. Beside, bioinformatic analysis revealed that HuR expression was inversely correlated with miR-145 expression in GC tissue samples and HuR was identified as a direct target of miR-145 with the dual-luciferase reporter. Enforced expression of miR-145 inhibited the HuR expression at both mRNA and protein levels as well as induced a similar biologic effects of silencing HuR in GC cells. Additional, we also found that restoration of HuR could eliminate the effects induced by miR-145 in GC cells. Taken together, these findings demonstrate the exact role of miR-145-HuR axis in the prog...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research