Pulsed high-power microwaves do not impair the functions of skin normal and cancer cells in vitro: a short-term biological evaluation

Publication date: Available online 20 November 2019Source: Journal of Advanced ResearchAuthor(s): Sohail Mumtaz, Pradeep Bhartiya, Neha Kaushik, Manish Adhikari, Pradeep Lamichhane, Su-Jae Lee, Nagendra Kumar Kaushik, Eun Ha ChoiAbstractOver the past few decades, microwave (MW) radiation has been widely used, and its biological effects have been extensively investigated. However, the effect of MW radiation on human skin biology is not well understood. We study the effects of pulsed high-power microwaves (HPMs) on melanoma (G361 and SK-Mel-31) and normal human dermal fibroblast (NHDF) cells. A pulsed power generator (Chundoong) was used to generate pulsed HPMs (dominant frequency: 3.5 GHz). For treatment 1, 5, 15, and 45 shots are given to cells in which the electromagnetic energy of 0.6 J was delivered to the cells at each trigger shot. Cell viability, proliferation rate, apoptosis, cell death, metabolic activity, and oxygen-free radical regulation were evaluated after the MW exposure at low and high doses. MW exposure increased the viabilities and proliferation rates of both melanoma cell lines in a dose-dependent manner, while no significant effects on the fibroblast cells were observed. We found an elevated level of ATP and mitochondrial activity in melanoma cells. Also, it was observed that MW exposure did not affect cell death in melanoma and fibroblast cells. A polymerase chain reaction analysis indicated that the MWs induced dose-dependent proliferation markers without...
Source: Journal of Advanced Research - Category: Research Source Type: research

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Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news
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Source: Journal of Immunotherapy - Category: Allergy & Immunology Tags: Review Articles Source Type: research
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Source: Journal of Immunotherapy - Category: Allergy & Immunology Tags: Clinical Studies Source Type: research
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Source: The Scientist - Category: Science Tags: Image of the Day Source Type: news
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
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Source: Seminars in Cancer Biology - Category: Cancer & Oncology Source Type: research
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Source: Life Sciences - Category: Biology Source Type: research
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