Viruses, Vol. 11, Pages 1051: Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms

Viruses, Vol. 11, Pages 1051: Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms Viruses doi: 10.3390/v11111051 Authors: Gaurav Rawal Franco Matias Ferreyra Nubia R. Macedo Laura K. Bradner Karen M. Harmon Adam Mueller Grant Allison Daniel C. L. Linhares Bailey L. Arruda Astroviruses cause disease in a variety of species. Yet, little is known about the epidemiology of a majority of astroviruses including porcine astrovirus type 3 (PoAstV3), which is a putative cause of polioencephalomyelitis in swine. Accordingly, a cross-sectional study was conducted on sow farms with or without reported PoAstV3-associated neurologic disease in growing pigs weaned from those farms. Additionally, a conveniently selected subset of piglets from one farm was selected for gross and histologic evaluation. The distribution of PoAstV3 in the enteric system was evaluated through in situ hybridization. PoAstV3, as detected by RT-qPCR on fecal samples, was frequently detected across sows and piglets (66–90%) on all farms (65–85%). PoAstV3 was detected subsequently at a similar detection frequency (77% vs 85%) on one farm after three months. Viral shedding, as determined by the cycle quantification value, suggests that piglets shed higher quantities of virus than adult swine. No link between gastrointestinal disease and PoAstV3 was found. However, PoAstV3 was detected by in situ in myenteric plexus neurons of piglets elucidat...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research