Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications

In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur. Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers. The scaffolds were evaluated for rheological properties – porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites. FTIR analysis showed intense peaks ranging 1120 -11267 cm−1 in the three different scaffolds that are denoted as CH groups. In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%). In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research

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Publication date: Available online 16 November 2019Source: Carbohydrate PolymersAuthor(s): Hossein Rahmani, Seyed Heydar Mahmoudi Najafi, Alireza Ashori, Marzieh Arab Fashapoyeh, Farzaneh Aziz Mohseni, Sara TorkamanAbstractIn this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided. The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross linker and the formation of urea linkage on mechanical properties such as tensile strength and elongation and dynamic-mechanical properties such as storage mod...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 16 November 2019Source: Journal of Environmental Chemical EngineeringAuthor(s): Mustapha Isah, Muhammad Hariz Asraf, Nik Ahmad Nizam Nik Malek, Khairunadwa Jemon, Nor Suriani Sani, Mustapha Salisu Muhammad, Mohd Firdaus Abdul Wahab, Mohammad Abdul Razis SaidinAbstractAccess to the portable drinking water is of paramount importance and remains major challenge globally. Water vending machine (WVM) could supplies drinking water to the community but the bacterial growth in WVM is of great health risk requires urgent attention. Chlorhexidine zinc-modified kaolinite (C-Zn-Kao-t1) was prepared, ...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
Publication date: Available online 5 November 2019Source: Inorganica Chimica ActaAuthor(s): Mousumi Nayak, Ashish Kumar Singh, Pradyot Prakash, Rajni Kant, Subrato BhattacharyaAbstractThiosalicylato complexes of Zn(II) and Hg(II), [Zn(SC6H4CO2)(TMEDA)]2 (1) and [TMEDA-H2][Hg(SC6H4CO2)2] (2) where (TMEDA = N,N,N',N'-tetramethylethylenediamine) have been synthesized and characterized by spectral and single crystal X-ray analyses. Thiosalicylate opted different binding modes; in 1 all three binding sites are used whereas in 2 bonding of the ligands are mainly through S atoms, however, Hg-O distances indicate the existence...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.
Source: Current Chemical Biology - Category: Biochemistry Source Type: research
Publication date: Available online 12 November 2019Source: Materials LettersAuthor(s): Li-Jun He, Jian-Chao Hao, Lin Dai, Rong-Chang Zeng, Shuo-Qi LiAbstractTo reduce the bacteria-associated infection for the Ti implant, an antibacterial coating composed of gentamicin (GS) and polyacrylic acid (PAA) was constructed using layer-by-layer (LbL) assembly technique. The surface morphology and chemical composition of the obtained multilayers were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The plate ...
Source: Materials Letters - Category: Materials Science Source Type: research
The quorum sensing (QS) system controls bacterial biofilm formation, which is highly related to the virulence and resistance of pathogens. In the present study, the effect of two traditional Chinese medicine (TCM) monomers, berberine and matrine, on biofilm formation and QS-related gene expression of antimicrobial-resistant (AMR) Escherichia coli strains was investigated by laser scanning confocal microscopy (LSCM) observation and real-time PCR. The results indicated a roughly positive relationship between biofilm formation ability and antimicrobial resistance. LSCM observation showed that berberine and matrine inhibited b...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract The study investigated the efficacy of two GRAS-status phytochemicals, mega-resveratrol (RV) and naringenin (NG) to inactivate Escherichia coli O157:H7 (EHEC) in apple cider. A five-strain mixture of EHEC (∼7 log CFU/ml) was inoculated into cider, followed by the addition of RV (8.7 mM and 13.0 mM) or NG (7.3 mM and 11.0 mM). The cider samples were stored at 4 °C for 14 days and EHEC was enumerated on days 0,1,5,7 and 14. The deleterious effects of RV and NG on EHEC cells were visualized by scanning electron microscopy (SEM), and RT-qPCR was done to determine the effect of phytochemicals...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
In this study, puroindoline A (PINA) protein was expressed in Escherichia coli, purified, and its antifungal properties against A. flavus were characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the molecular weight of the recombinant PINA protein was approximately 44 kDa. PINA exerted a powerful antifungal effect against A. flavus at 42.42 μg/mL on potato dextrose agar culture medium. Flow cytometry and scanning electron microscopy revealed that the spore morphology was damaged by PINA exposure; spores were depressed and broken, suggesting that the cell wall was impaired. Transmission e...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts. Mol Med Rep. 2019 Sep 25;: Authors: Kong F, Cai X, Zhai S, Wang R, Zheng X, Ma Y, Bi H, Wang D Abstract The present study aimed to evaluate the antimicrobial activity and the possible mechanisms of activity of polypeptide‑enriched Gastrodia elata extracts (GEP) against the gram‑negative bacteria Escherichia coli and Pseudomonas aeruginosa, the gram‑positive bacterium Staphylococcus aureus and the fungus Candida albicans. The antimicrobial activity of GEP was first confirmed by determining th...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Abstract Copper nanoparticles were synthesized via precipitation technique using the pseudonatural cationic chitosan biopolymer as a stabilizing agent. The nanoparticles developed were successfully incorporated into the 1:1 ratio of blended chitosan: pluronic F127 polymer solution and made their nanocomposite hydrogels by solution casting method. The formed copper-based nanocomposite hydrogels were characterized by using Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy and transmission electron microscopy studies. Th...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
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