Rapid extraction method followed by a d-SPE clean-up step for determination of phenolic composition and antioxidant and antiproliferative activities from berry fruits

Publication date: Available online 19 October 2019Source: Food ChemistryAuthor(s): Carina Alexandra Rodrigues, Antonio Eduardo Nicácio, Joana Schuelter Boeing, Franciele Pelegrin Garcia, Celso Vataru Nakamura, Jesuí Vergílio Visentainer, Liane MaldanerAbstractDespite the numerous studies that have shown a wide range of biological activities to berry fruits, scientific data focusing on modern, rapid and simple extraction methods followed by a clean-up step is still lacking. Therefore, the present work was aimed at investigating the use of a fast one-step liquid–solid extraction procedure followed by a dispersive solid-phase extraction (d-SPE) clean-up step to evaluate the phenolic composition, antioxidant and antiproliferative activities from three of the principal berries found in Brazil, pomegranate (Punica granatum L.), blackberry (Rubus ulmifolius Schott.), and strawberry (Fragaria x ananassa Duch.). Under the optimized extraction conditions, sixteen phenolic compounds were determined by UHPLC-MS/MS analysis and all berry extracts showed antioxidant activity and antiproliferative effects on cervical (HeLa) and colon (HT-29) cancer cells. Overall, these results highlight the importance of the clean-up step for more reliable data in studies of health-promoting proprieties from berry fruits.
Source: Food Chemistry - Category: Food Science Source Type: research