Tributyltin Reduces Bone Mineral Density by Reprograming Bone Marrow Mesenchymal Stem Cells in Rat

Publication date: Available online 7 October 2019Source: Environmental Toxicology and PharmacologyAuthor(s): Wenhuan Yao, Xinglong Wei, Hao Guo, Dong Cheng, Hui Li, Limin Sun, Shu’e Wang, Dongmei Guo, Yanli Yang, Jiliang SiAbstractTributyltin (TBT), a proven endocrine disrupter, was widely used in industry and agriculture. Previous research showed that TBT could alter the balance between osteogenesis and adipogenesis, which may have significant consequences for bone health. Herein, we exposed male rats to TBT chloride (TBTCl) to evaluate the deleterious effects of TBT on bone. Exposure to 50 μg kg-1 TBT resulted in a significant decrease in bone mineral density (BMD) at the femur diaphysis region in the rat. A dose-dependent increase in lipid accumulation and adipocyte number was observed in the bone marrow (BM) of the femur. Meanwhile, TBTCl treatment significantly enhanced the expression of PPARγ and attenuated the expression of Runx2 and β-catenin in BM. In addition, serum ALP activity of TBT-exposed rats also showed a dose-dependent decrease. These results suggest that TBT could reduce BMD via inhibition of the Wnt/β-catenin pathway and skew the adipo-osteogenic balance in the BM of rats.Graphical abstract
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research