Dual roles of juvenile hormone signaling during early oogenesis in Drosophila

AbstractJuvenile hormone (JH) signaling plays crucial roles in insect metamorphosis and reproduction. Function of JH signaling in germline stem cells (GSCs) remains largely unknown. Here, we found that the number of GSCs significantly declined in the ovaries ofMet,Gce andJHAMT mutants. Then we inhibited JH signaling in selected cell types of ovaries by expressingMet andGce orKr ‐h1 double ‐stranded RNAs (dsRNAs) using different Gal4 drivers. Blocking of JH signaling in muscle cells has no effect on GSC numbers. Blocking of JH signaling in cap cells reduced GSCs cells. Inductive expression ofMet andGce dsRNA but notKr ‐h1 by Nos ‐Gal4 increased GSC cells. These results indicate that JH signaling plays an important role in GSC maintenance.
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research