Molecular characteristics of novel mono-reassortant G9P[8] rotavirus A strains possessing the NSP4 gene of the E2 genotype detected in Tokyo, Japan.

Molecular characteristics of novel mono-reassortant G9P[8] rotavirus A strains possessing the NSP4 gene of the E2 genotype detected in Tokyo, Japan. Jpn J Infect Dis. 2019 Sep 30;: Authors: Fujii Y, Oda M, Somura Y, Shinkai T Abstract Rotavirus A (RVA) has been detected from patients with gastroenteritis even after vaccine introduction in Japan. To investigate the circulating RVA strains, the RVA-positive stool specimens obtained in Tokyo in 2017 and 2018 were analyzed by next-generation sequencing. A total of 50 and 21 RVA samples were obtained in 2017 and 2018, respectively. In 2017, G2P[4] (40.0%) was the most prevalent strain, followed by G3P[8] (DS-1-like) (28.0%), G8P[8] (10.0%), G3P[8] (Wa-like) (8.0%), G9P[8]-E1 (8.0%), and mixed infection (6.0%). In 2018, G3P[8] (DS-1-like) (28.6%) and G9P[8]-E2 (28.6%) were the prevalent strains, followed by G9P[8]-E1 (19.0%), G2P[4] (9.5%), G8P[8] (9.5%), and mixed infection (4.8%). Six G9P[8]-E2 strains detected in 2018 were atypical genotype constellation (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1), which has not been reported. On phylogenetic analyses, this virus was considered to be generated by inter-genogroup reassortment between commonly circulating G9P[8] and G2P[4] strains in Japan. The G9P[8] strain seemed to be reassorted with only the NSP4 gene of the E2 genotype of the G2P[4] strain. Since this newly-emerged G9P[8]-E2 virus was detected in different locations in Tokyo, this virus see...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research