Evaluation of the occurrence of sporulating and nonsporulating pathogenic bacteria in manure and in digestate of five agricultural biogas plants

In this study, three replicates of manure and digestate were collected from five farm biogas plants receiving animal manure in order to assess the occurrence and concentrations of sporulating (Clostridium botulinum,Clostridioides difficile,Clostridium perfringens) and nonsporulating (Listeria monocytogenes, thermotolerantCampylobacter spp.,Salmonella,Escherichia coli, enterococci) bacteria. Concentrations ofE. coli, enterococci, andC. perfringens in digestates ranged from 102 to 104, 104 to 105, and
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research

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The objective of this study was to evaluate the effectiveness of pulsed light (PL) treatment, a novel antimicrobial (LAPEN) wash and combinations thereof in inactivating Salmonella on stem scars of cherry tomato. The treatment effects on background microbiota and sensory quality was also investigated while in storage for 21 days at 10 °C. Three serotypes of Salmonella enterica were chosen to prepare inoculum for the current investigation for their link with tomato and produce outbreaks. Stem scars of tomato were spot inoculated before being treated with PL (1–63 J/cm2), LAPEN sanitizer (2 min) or combinatio...
Source: Food Control - Category: Food Science Source Type: research
CONCLUSIONS: The matrices and the questionnaire have a great diagnostic performance which seems interesting for a use as a screening tool for occupational exposures. These results have yet to be confirmed by large-scale studies. PMID: 31727556 [PubMed - as supplied by publisher]
Source: Revue des Maladies Respiratoires - Category: Respiratory Medicine Tags: Rev Mal Respir Source Type: research
Publication date: Available online 16 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Biao Zhang, Lili Ren, Dayong Xu, Haonan Wang, Zhuo Chen, Bingrong Zhang, Xin Zeng, Lianhong Sun, Feng LiAbstractQuorum sensing is a population density-dependent gene expression regulation mechanism in bacteria. The substrate specificity of RhlI, an enzyme in the RhlI–RhlR quorum sensing system of Pseudomonas aeruginosa, was explored by directed evolution to gain insight into the molecular mechanisms of quorum sensing. RhlI catalyzes S-adenosyl methionine and butanoyl or hexanoyl acyl carrier protein to form N-butanoyl...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Publication date: November 2019Source: Saudi Journal of Biological Sciences, Volume 26, Issue 7Author(s): Priyanka Verma, Ajar Nath Yadav, Kazy Sufia Khannam, Shashank Mishra, Sanjay Kumar, Anil Kumar Saxena, Archna SumanAbstractThe biodiversity of wheat associated bacteria was deciphered from the peninsular zone of India. A total of 264 isolated bacteria were analyzed through amplified ribosomal DNA restriction analysis (ARDRA, using three restriction enzymes Alu I, Msp I and Hae III, which led to the clustering of these isolates into 12–16 groups for the different sites at>75% similarity index, adding up to 70 g...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Publication date: Available online 15 November 2019Source: Biological ControlAuthor(s): N-N Wang, K-Y Gao, N Han, R-Z Tian, J-L Zhang, X Yan, L-L HuangAbstractApple Valsa canker (AVC), caused by the fungus Valsa mali (Vm), is a serious and persistent disease affecting apple production in China that is difficult to control chemically and agriculturally. In order to develop biological control agents (BCAs) for this disease, an antifungal activity-deficient Bacillus amyloliquefaciens mutant, ED-2544, was characterized using PCR amplification, Southern blotting, and SiteFinding-PCR. The B. amyloliquefaciens ChbB(chitin-binding...
Source: Biological Control - Category: Biology Source Type: research
Publication date: Available online 16 November 2019Source: Carbohydrate PolymersAuthor(s): Hossein Rahmani, Seyed Heydar Mahmoudi Najafi, Alireza Ashori, Marzieh Arab Fashapoyeh, Farzaneh Aziz Mohseni, Sara TorkamanAbstractIn this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided. The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross linker and the formation of urea linkage on mechanical properties such as tensile strength and elongation and dynamic-mechanical properties such as storage mod...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 15 November 2019Source: Protein Expression and PurificationAuthor(s): Balabhadra Khatiwada, Jeffrey A. Purslow, Eric S. Underbakke, Vincenzo VendittiAbstractVarious fusion tags are commonly employed to increase the heterologous expression and solubility of aggregation-prone proteins within Escherichia coli. Herein, we present a protocol for efficient recombinant expression and purification of the human RNA demethylases Alkbh5 and FTO. Our method incorporates a novel fusion tag (the N-terminal domain of bacterial enzyme I, EIN) that dramatically increases the solubility of its fusion partn...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Carolina Medina Amado, Carlos J. Minahk, Eduardo Cilli, Rafael G. Oliveira, Fernando G. DupuyAbstractThe mechanism of action of the anti-Listeria peptide enterocin CRL35 was studied with biophysical tools by using lipid mixtures that mimicked Gram-positive plasma membranes. Langmuir monolayers and infrared spectroscopy indicated that the peptide readily interacted with phospholipid assembled in monolayers and bilayers to produce a dual effect, depending on the acyl chains. Indeed, short chain mixtures wer...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
Publication date: Available online 16 November 2019Source: Journal of Environmental Chemical EngineeringAuthor(s): Mustapha Isah, Muhammad Hariz Asraf, Nik Ahmad Nizam Nik Malek, Khairunadwa Jemon, Nor Suriani Sani, Mustapha Salisu Muhammad, Mohd Firdaus Abdul Wahab, Mohammad Abdul Razis SaidinAbstractAccess to the portable drinking water is of paramount importance and remains major challenge globally. Water vending machine (WVM) could supplies drinking water to the community but the bacterial growth in WVM is of great health risk requires urgent attention. Chlorhexidine zinc-modified kaolinite (C-Zn-Kao-t1) was prepared, ...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
Publication date: Available online 15 November 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): C. Vijilvani, M.R. Bindhu, F.C. Frincy, Mohamad S. AlSalhi, S. Sabitha, K. Saravanakumar, Sandhanasamy Devanesan, M. Umadevi, Mamduh J. Aljaafreh, Muhammad AtifAbstractThe field of nanobiotechnology and nanomedicine paves way for the use of several nanoparticles. Especially, in biomedical applications, the silver nanoparticles (AgNPs), gold nanoparticles (AuNPs) and palladium nanoparticles (PdNPs) are found most vital and promising, among other nanoparticles. The biomedical activities of these particle...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
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